BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5532

Title: Structure of the Tsg101 UEV Domain in Complex with a HIV-1 PTAP "Late Domain" Peptide   PubMed: 12379843

Authors: Pornillos, O.; Alam, S.; Davis, D.; Sundquist, W.

Citation: Pornillos, O.; Alam, S.; Davis, D.; Sundquist, W.. "Structure of the Tsg101 UEV Domain in Complex with the PTAP Motif of the HIV-1 p6 Protein"  Nat. Struct. Biol. 9, 812-817 (2002).

Assembly members:
Tumor Susceptibility gene 101 protein, polymer, 145 residues, Formula weight is not available
Gag, polymer, 9 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Tumor Susceptibility gene 101 protein: MAVSESQLKKMVSKYKYRDL TVRETVNVITLYKDLKPVLD SYVFNDGSSRELMNLTGTIP VPYRGNTYNIPICLWLLDTY PYNPPICFVKPTSSMTIKTG KHVDANGKIYLPYLHEWKHP QSDLLGLIQVMIVVFGDEPP VFSRP
Gag: PEPTAPPEE

Data sets:
Data typeCount
1H chemical shifts1059
13C chemical shifts646
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tumor Susceptibility gene 101 protein1
2Gag Polyprotein2

Entities:

Entity 1, Tumor Susceptibility gene 101 protein 145 residues - Formula weight is not available

1   METALAVALSERGLUSERGLNLEULYSLYS
2   METVALSERLYSTYRLYSTYRARGASPLEU
3   THRVALARGGLUTHRVALASNVALILETHR
4   LEUTYRLYSASPLEULYSPROVALLEUASP
5   SERTYRVALPHEASNASPGLYSERSERARG
6   GLULEUMETASNLEUTHRGLYTHRILEPRO
7   VALPROTYRARGGLYASNTHRTYRASNILE
8   PROILECYSLEUTRPLEULEUASPTHRTYR
9   PROTYRASNPROPROILECYSPHEVALLYS
10   PROTHRSERSERMETTHRILELYSTHRGLY
11   LYSHISVALASPALAASNGLYLYSILETYR
12   LEUPROTYRLEUHISGLUTRPLYSHISPRO
13   GLNSERASPLEULEUGLYLEUILEGLNVAL
14   METILEVALVALPHEGLYASPGLUPROPRO
15   VALPHESERARGPRO

Entity 2, Gag Polyprotein 9 residues - Formula weight is not available

1   PROGLUPROTHRALAPROPROGLUGLU

Samples:

sample_1: Tumor Susceptibility gene 101 protein 1.5 mM; Gag 1.5 mM

sample_cond_1: pH: 5.5; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
15N/1H FHSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
13C/1H CT-HSQCnot availablenot availablenot available
3D 15N-edited TOCSY-HSQCnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
(H)C(CO)NHnot availablenot availablenot available
3D 13C-edited NOESY-HSQCnot availablenot availablenot available
3D 15N-edited NOESY-HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available
F1-edited/F2-filtered NOESY-HSQCnot availablenot availablenot available

Software:

DYANA v1.2 - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SWISS-PROT Q99816
REF XP_508319 XP_001505015 XP_001173373 NP_006283 NP_001091464
GenBank AAX36281 AAX32198 AAI33616 AAH02487 AAC52083
EMBL CAH90615
PDB