BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5538

Title: 1H, 13C, and 15N Chemical Shift Assignments for a PWWP domain from Sch. pombe   PubMed: 12842472

Authors: Slater, Leanne; Allen, Mark; Bycroft, Mark

Citation: Slater, Leanne; Allen, Mark; Bycroft, Mark. "Structural Variation in PWWP Domains"  J. Mol. Biol. 330, 571-576 (2003).

Assembly members:
PWWP domain, polymer, 109 residues, 12194 Da.

Natural source:   Common Name: Fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PWWP domain: GSERVNYKPGMRVLTKMSGF PWWPSMVVTESKMTSVARKS KPKRAGTFYPVIFFPNKEYL WTGSDSLTPLTSEAISQFLE KPKPKTASLIKAYKMAQSTP DLDSLSVPS

Data sets:
Data typeCount
1H chemical shifts787
13C chemical shifts364
15N chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PWWP domain1

Entities:

Entity 1, PWWP domain 109 residues - 12194 Da.

1   GLYSERGLUARGVALASNTYRLYSPROGLY
2   METARGVALLEUTHRLYSMETSERGLYPHE
3   PROTRPTRPPROSERMETVALVALTHRGLU
4   SERLYSMETTHRSERVALALAARGLYSSER
5   LYSPROLYSARGALAGLYTHRPHETYRPRO
6   VALILEPHEPHEPROASNLYSGLUTYRLEU
7   TRPTHRGLYSERASPSERLEUTHRPROLEU
8   THRSERGLUALAILESERGLNPHELEUGLU
9   LYSPROLYSPROLYSTHRALASERLEUILE
10   LYSALATYRLYSMETALAGLNSERTHRPRO
11   ASPLEUASPSERLEUSERVALPROSER

Samples:

sample_1: PWWP domain, [U-99% 13C; U-99% 15N], 1.0 mM; potassium phosphate 50 mM; NaCl 300 mM

Ex-cond_1: pH: 6.0; temperature: 290 K; ionic strength: 0.35 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D DQF-COSYnot availablenot availablenot available
15N-HSQCnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNHBnot availablenot availablenot available
13C-NOESYnot availablenot availablenot available

Software:

Ansig v3.3 - assignment, through space NOE determination

NMR spectrometers:

  • Bruker Advance 800 MHz
  • Bruker Advance 600 MHz

Related Database Links:

PDB
EMBL CAA22121
REF NP_596684
SP O94312