BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5554

Title: Structure of the N-WASP EVH1 Domain-WIP complex   PubMed: 12437929

Authors: Volkman, B.; Prehoda, K.; Scott, J.; Peterson, F.; Lim, W.

Citation: Volkman, B.; Prehoda, K.; Scott, J.; Peterson, F.; Lim, W.. "Structure of the N-WASP EVH1 Domain-WIP Complex: Insight into the Molecular Basis of Wiskott-Aldrich Syndrome "  Cell 111, 565-576 (2002).

Assembly members:
WIP PEPTIDE AND N-WASP EVH1 DOMAIN, polymer, 144 residues, Formula weight is not available

Natural source:   Common Name: black rat   Taxonomy ID: 10117   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: rattus rattus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
WIP PEPTIDE AND N-WASP EVH1 DOMAIN: DLPPPEPYVQTTKSYPSKLA RNESRGSGSGSLFSFLGKKC VTMSSAVVQLYAADRNCMWS KKCSGVACLVKDNPQRSYFL RIFDIKDGKLLWEQELYNNF VYNSPRGYFHTFAGDTCQVA LNFANEEEAKKFRKAVTDLL GRRQ

Data sets:
Data typeCount
1H chemical shifts848
13C chemical shifts522
15N chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Wiskott-Aldrich syndrome protein interacting protein plus link plus WASP1

Entities:

Entity 1, Wiskott-Aldrich syndrome protein interacting protein plus link plus WASP 144 residues - Formula weight is not available

1   ASPLEUPROPROPROGLUPROTYRVALGLN
2   THRTHRLYSSERTYRPROSERLYSLEUALA
3   ARGASNGLUSERARGGLYSERGLYSERGLY
4   SERLEUPHESERPHELEUGLYLYSLYSCYS
5   VALTHRMETSERSERALAVALVALGLNLEU
6   TYRALAALAASPARGASNCYSMETTRPSER
7   LYSLYSCYSSERGLYVALALACYSLEUVAL
8   LYSASPASNPROGLNARGSERTYRPHELEU
9   ARGILEPHEASPILELYSASPGLYLYSLEU
10   LEUTRPGLUGLNGLULEUTYRASNASNPHE
11   VALTYRASNSERPROARGGLYTYRPHEHIS
12   THRPHEALAGLYASPTHRCYSGLNVALALA
13   LEUASNPHEALAASNGLUGLUGLUALALYS
14   LYSPHEARGLYSALAVALTHRASPLEULEU
15   GLYARGARGGLN

Samples:

sample_1: WIP PEPTIDE AND N-WASP EVH1 DOMAIN, [U-15N], 1 mM; phosphate buffer 20 mM; dithiothreitol 1 mM; H2O 90%; D2O 10%

sample_2: WIP PEPTIDE AND N-WASP EVH1 DOMAIN, [U-15N; U-13C], 1 mM; phosphate buffer 20 mM; dithiothreitol 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7.0; temperature: 303 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

DYANA v1.5 - structure solution, refinement

XWINNMR v3.0 - collection

xeasy v1.3 - data analysis

NMRPipe - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA21534 BAB29159 BAE24270 BAE25143 BAE25639
EMBL CAC69994
GB AAH19951 AAH55045 AAH58642 EAL24333 EDL13819
REF NP_001103835 NP_001161217 NP_082735 XP_002712136 XP_003134773
SP O08816 Q91YD9