BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5556

Title: PHD Fingers as Protein Scaffolds   PubMed: 12842043

Authors: Kwan, A.; Matthews, J.; Mackay, J.

Citation: Kwan, A.; Gell, D.; Verger, A.; Crossley, M.; Matthews, J.; Mackay, J.. "Engineering a Protein Scaffold from a PHD Finger"  Structure 11, 803-813 (2003).

Assembly members:
PHD, polymer, 61 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PHD: GPLGSDHHMEFCRVCKDGGE LLCCDTCPSSYHIHCLRPAL YEVPDGEWQCPRCTCPALKG K

Data sets:
Data typeCount
1H chemical shifts311

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mi2-beta with WSTF1
2ZINC ION2

Entities:

Entity 1, Mi2-beta with WSTF 61 residues - Formula weight is not available

1   GLYPROLEUGLYSERASPHISHISMETGLU
2   PHECYSARGVALCYSLYSASPGLYGLYGLU
3   LEULEUCYSCYSASPTHRCYSPROSERSER
4   TYRHISILEHISCYSLEUARGPROALALEU
5   TYRGLUVALPROASPGLYGLUTRPGLNCYS
6   PROARGCYSTHRCYSPROALALEULYSGLY
7   LYS

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: PHD 1 mM; DTT 1 mM; sodium phosphate buffer 10 mM; NaCl 150 mM; H2O 95%; D2O 5%

sample_cond_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1

Software:

xwinnmr v2.5 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - refinement

ARIA v1.1.2 - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

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