BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5565

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for porcine MSP

Authors: Wang, Iren; Lou, Yuan-Chao; Wu, Kuen-Phon; Wu, Shih-Hsiung; Chang, Wen-Chang; Chen, Chinpan

Citation: Wang, Iren; Lou, Yuan-Chao; Wu, Kuen-Phon; Wu, Shih-Hsiung; Chang, Wen-Chang; Chen, Chinpan. "Disulfide pairings and structure of porcine beta-microseminoprotein"  The BMRB entry is the only known published source for the data..

Assembly members:
beta-microseminoprotein, polymer, 91 residues, Formula weight is not available

Natural source:   Common Name: Pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
beta-microseminoprotein: QCYFIPNQSLKPNECQDLKG VSHPLNSVWKTKDCEECTCG QDAISCCNTAAIPTGYDTNK CQKILNKKTCTYTVVEKKDP GKTCDVTGWVL

Data sets:
Data typeCount
1H chemical shifts603
13C chemical shifts394
15N chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1porcine MSP1

Entities:

Entity 1, porcine MSP 91 residues - Formula weight is not available

1   GLNCYSTYRPHEILEPROASNGLNSERLEU
2   LYSPROASNGLUCYSGLNASPLEULYSGLY
3   VALSERHISPROLEUASNSERVALTRPLYS
4   THRLYSASPCYSGLUGLUCYSTHRCYSGLY
5   GLNASPALAILESERCYSCYSASNTHRALA
6   ALAILEPROTHRGLYTYRASPTHRASNLYS
7   CYSGLNLYSILELEUASNLYSLYSTHRCYS
8   THRTYRTHRVALVALGLULYSLYSASPPRO
9   GLYLYSTHRCYSASPVALTHRGLYTRPVAL
10   LEU

Samples:

Sample_1: beta-microseminoprotein, [U-13C; U-15N], 1.5 mM

Ex-cond_1: pH: 5.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

XWINNMR v2.5 - data processing

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 7243 7304
PDB
GB AAB50711
REF NP_999017
SP O02826