BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5570

Title: 1H, 13C, and 15N NMR assignments of the hypothetical Nudix protein DR0079 from the extremely radiation-resistant bacterium Deinococcus radiodurans

Authors: Buchko, Garry; Ni, Shusoing; Holbrook, Stephen; Kennedy, Michael

Citation: Buchko, Garry; Ni, Shusoing; Holbrook, Stephen; Kennedy, Michael. "Letter to the Editor: 1H, 13C, and 15N NMR assignments of the hypothetical Nudix protein DR0079 from the extremely radiation-resistant bacterium Deinococcus radiodurans"  J. Biomol. NMR 25, 169-170 (2003).

Assembly members:
DR0079, polymer, 171 residues, 19283 Da.

Natural source:   Common Name: D. radiodurans   Taxonomy ID: 1299   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Deinococcus radiodurans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DR0079: MGGVSDERLDLVNERDEVVG QILRTDPALRWERVRVVNAF LRNSQGQLWIPRRSPSKSLF PNALDVSVGGAVQSGETYEE AFRREAREELNVEIDALSWR PLASFSPFQTTLSSFMCVYE LRSDATPIFNPNDISGGEWL TPEHLLARIAAGEAAKGDLA ELVRRCYREEE

Data sets:
Data typeCount
1H chemical shifts917
13C chemical shifts580
15N chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DR00791

Entities:

Entity 1, DR0079 171 residues - 19283 Da.

1   METGLYGLYVALSERASPGLUARGLEUASP
2   LEUVALASNGLUARGASPGLUVALVALGLY
3   GLNILELEUARGTHRASPPROALALEUARG
4   TRPGLUARGVALARGVALVALASNALAPHE
5   LEUARGASNSERGLNGLYGLNLEUTRPILE
6   PROARGARGSERPROSERLYSSERLEUPHE
7   PROASNALALEUASPVALSERVALGLYGLY
8   ALAVALGLNSERGLYGLUTHRTYRGLUGLU
9   ALAPHEARGARGGLUALAARGGLUGLULEU
10   ASNVALGLUILEASPALALEUSERTRPARG
11   PROLEUALASERPHESERPROPHEGLNTHR
12   THRLEUSERSERPHEMETCYSVALTYRGLU
13   LEUARGSERASPALATHRPROILEPHEASN
14   PROASNASPILESERGLYGLYGLUTRPLEU
15   THRPROGLUHISLEULEUALAARGILEALA
16   ALAGLYGLUALAALALYSGLYASPLEUALA
17   GLULEUVALARGARGCYSTYRARGGLUGLU
18   GLU

Samples:

sample_1: DR0079, [U-15N; U-13C], 0.1 – 4 mM

condition_1: pH: 7.1 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQC-NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CCC-TOCSY-NNHnot availablenot availablenot available
CBCACOCAHAnot availablenot availablenot available

Software:

FELIX v97 - data processing

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
GB AAF09672
REF NP_293805 WP_010886727
SP Q9RY71