BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5579

Title: Two Homologous Rat Cellular Retinol-binding Proteins Differ in Local Conformational Flexibility   PubMed: 12850148

Authors: Lu, Jianyun; Cistola, David; Li, Ellen

Citation: Lu, Jianyun; Cistola, David; Li, Ellen. "Two Homologous Rat Cellular Retinol-binding Proteins Differ in Local Conformational Flexibility"  J. Mol. Biol. 330, 799-812 (2003).

Assembly members:
rat cellular retinol-binding protein I, polymer, 134 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
rat cellular retinol-binding protein I: PVDFNGYWKMLSNENFEEYL RALDVNVALRKIANLLKPDK EIVQDGDHMIIRTLSTFRNY IMDFQVGKEFEEDLTGIDDR KCMTTVSWDGDKLQCVQKGE KEGRGWTQWIEGDELHLEMR AEGVTCKQVFKKVH

Data sets:
Data typeCount
1H chemical shifts940
13C chemical shifts551
15N chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-CRBP I1

Entities:

Entity 1, apo-CRBP I 134 residues - Formula weight is not available

1   PROVALASPPHEASNGLYTYRTRPLYSMET
2   LEUSERASNGLUASNPHEGLUGLUTYRLEU
3   ARGALALEUASPVALASNVALALALEUARG
4   LYSILEALAASNLEULEULYSPROASPLYS
5   GLUILEVALGLNASPGLYASPHISMETILE
6   ILEARGTHRLEUSERTHRPHEARGASNTYR
7   ILEMETASPPHEGLNVALGLYLYSGLUPHE
8   GLUGLUASPLEUTHRGLYILEASPASPARG
9   LYSCYSMETTHRTHRVALSERTRPASPGLY
10   ASPLYSLEUGLNCYSVALGLNLYSGLYGLU
11   LYSGLUGLYARGGLYTRPTHRGLNTRPILE
12   GLUGLYASPGLULEUHISLEUGLUMETARG
13   ALAGLUGLYVALTHRCYSLYSGLNVALPHE
14   LYSLYSVALHIS

Samples:

sample_1: rat cellular retinol-binding protein I, [U-99% 15N; U-13C], 1.0 mM

Sample_2: rat cellular retinol-binding protein I, [U-80% 2H; U-99% 15N], 1.0 mM

Sample_3: rat cellular retinol-binding protein I, [U-99% 15N], 1.0 mM

Ex-cond_1: pH: 7.4; temperature: 298 K

Ex-cond_2: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONNHnot availablenot availablenot available
CBCACOCAHAnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available

Software:

FELIX v2000 - automatic peak picking

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian UNITYPLUS 500 MHz
  • Varian UNITY 600 MHz

Related Database Links:

BMRB 5048 5319 5330 5331 5578
PDB
DBJ BAB27753
EMBL CAA42919
GB AAA35714 AAA40962 AAA42021 AAH18254 AAH91751
REF NP_035384 NP_036865 XP_005080789 XP_005366741 XP_006083819
SP P02696 Q00915