BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5581

Title: NMR solution structure of type-B lantibiotics mersacidin in MeOH/H2O mixture   PubMed: 12562773

Authors: Hsu, Shang-Te; Breukink, Eefjan; Bierbaum, Gabriele; Sahl, Hans-Georg; de Kruijff, Ben; Kaptein, Rob; van Nuland, Nico; Bonvin, Alexandre

Citation: Hsu, Shang-Te; Breukink, Eefjan; Bierbaum, Gabriele; Sahl, Hans-Georg; de Kruijff, Ben; Kaptein, Rob; van Nuland, Nico; Bonvin, Alexandre. "NMR Study of Mersacidin and Lipid II Interaction in Dodecylphosphocholine Micelles: Conformational Changes are a Key to Antimicrobial Activity"  J. Biol. Chem. 278, 13110-13117 (2003).

Assembly members:
Type B lantibiotics mersacidin, polymer, 20 residues, Formula weight is not available

Natural source:   Common Name: Bacillus sp.   Taxonomy ID: 1409   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus Bacillus sp.

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Type B lantibiotics mersacidin: CXFXLPGGGGVCXLXXECIX

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1lantibiotic Mersacidin1

Entities:

Entity 1, lantibiotic Mersacidin 20 residues - Formula weight is not available

1   CYSABAPHEABALEUPROGLYGLYGLYGLY
2   VALCYSABALEUABADHAGLUCYSILEDHL

Samples:

sample_1: Type B lantibiotics mersacidin 2 mM; MeOH, [U-2H], 37%

sample_cond_1: pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1

Software:

xwinnmr - collection

NMRPipe - processing

CNS v1.1 - structure solution

ARIA v1.1 - structure solution

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

BMRB 5580 5581 5582
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