BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5585

Title: Isolation, structure and activity of GID, a novel alpha4/7-conotoxin with an extended N-terminal sequence   PubMed: 12419800

Authors: Nicke, A.; Loughnan, M.; Millard, E.; Alewood, P.; Adams, D.; Daly, N.; Craik, D.; Lewis, R.

Citation: Nicke, A.; Loughnan, M.; Millard, E.; Alewood, P.; Adams, D.; Daly, N.; Craik, D.; Lewis, R.. "Isolation, Structure, and Activity of GID, a Novel Alpha 4/7-conotoxin with an Extended N-terminal Sequence"  J. Biol. Chem. 278, 3137-3144 (2003).

Assembly members:
alpha-conotoxin GID, polymer, 19 residues, Formula weight is not available

Natural source:   Common Name: geography cone   Taxonomy ID: 6491   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus Geographus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
alpha-conotoxin GID: IRDXCCSNPACRVNNXHVC

Data sets:
Data typeCount
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-conotoxin GID1

Entities:

Entity 1, alpha-conotoxin GID 19 residues - Formula weight is not available

1   ILEARGASPCGUCYSCYSSERASNPROALA
2   CYSARGVALASNASNHYPHISVALCYS

Samples:

sample_1: alpha-conotoxin GID 2.5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0 M; pH: 2.8; pressure: 1 atm; temperature: 287 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

XWINNMR v2.6 - collection

XEASY v1.3.7 - data analysis

DYANA v1.5 - structure solution

CNS v1.0 - refinement

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker ARX 500 MHz

Related Database Links:

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