BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5604

Title: Solution structure of the N-terminal domain of ZntA in the apo- and Zn(II) forms   PubMed: 12417201

Authors: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Finney, L.; Outten, C.; O'Halloran, T.

Citation: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Finney, L.; Outten, C.; O'Halloran, T.. "A new-zinc protein coordination site in intracellular metal trafficking: solution structure of the apo and Zn(II) forms of ZntA (46-118)"  J. Mol. Biol. 323, 883-897 (2002).

Assembly members:
p-type ATPase, polymer, 73 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
p-type ATPase: SGTRYSWKVSGMDCAACARK VENAVRQLAGVNQVQVLFAT EKLVVDADNDIRAQVESALQ KAGYSLRDEQAAE

Data sets:
Data typeCount
1H chemical shifts406
15N chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P-type zinc atpase1

Entities:

Entity 1, P-type zinc atpase 73 residues - Formula weight is not available

1   SERGLYTHRARGTYRSERTRPLYSVALSER
2   GLYMETASPCYSALAALACYSALAARGLYS
3   VALGLUASNALAVALARGGLNLEUALAGLY
4   VALASNGLNVALGLNVALLEUPHEALATHR
5   GLULYSLEUVALVALASPALAASPASNASP
6   ILEARGALAGLNVALGLUSERALALEUGLN
7   LYSALAGLYTYRSERLEUARGASPGLUGLN
8   ALAALAGLU

Samples:

sample_1: p-type ATPase, [U-15N], 1.5 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%

sample_2: p-type ATPase 2 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1

Software:

XWINNMR v1.3 - collection, processing

DYANA v1.5 - structure solution

CORMA - iterative matrix relaxation

AMBER v5.0 - refinement

xeasy v3.2 - data analysis

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
DBJ BAB37741 BAE77824 BAG79261 BAI32897 BAJ45202
EMBL CAQ90919 CAR00414 CAR15077 CAV00282 CBG36554
GB AAB18444 AAC76494 AAG58578 AAN44946 AAP19236
REF NP_290017 NP_312345 NP_417926 NP_709239 NP_839425
SP P37617