BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5607

Title: NMR Structure of the Extended Second Transmembrane Domain of the Human Neuronal Glycine Receptor alpha1 Subunit in SDS Micelles   PubMed: 12667090

Authors: Yushmanov, V.; Mandal, P.; Liu, Z.; Tang, P.; Xu, Y.

Citation: Yushmanov, V.; Mandal, P.; Liu, Z.; Tang, P.; Xu, Y.. "NMR Structure and Backbone Dynamics of the Extended Second Transmembrane Domain of the Human Neuronal Glycine Receptor alpha1 Subunit"  Biochemistry 42, 3989-3995 (2003).

Assembly members:
Human Neuronal Glycine Receptor alpha-1 Chain TM2, polymer, 28 residues, 2801.25 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Human Neuronal Glycine Receptor alpha-1 Chain TM2: APARVGLGITTVLTMTTQSS GSRASLPK

Data sets:
Data typeCount
1H chemical shifts157
15N chemical shifts25
coupling constants19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Glycine Receptor alpha-1 Chain TM2, I1
2Glycine Receptor alpha-1 Chain TM2, II1
3Glycine Receptor alpha-1 Chain TM2, III1
4Glycine Receptor alpha-1 Chain TM2, IV1

Entities:

Entity 1, Glycine Receptor alpha-1 Chain TM2, I 28 residues - 2801.25 Da.

1   ALAPROALAARGVALGLYLEUGLYILETHR
2   THRVALLEUTHRMETTHRTHRGLNSERSER
3   GLYSERARGALASERLEUPROLYS

Samples:

sample_1: Human Neuronal Glycine Receptor alpha-1 Chain TM2, [U-15N], 2 mM; sodium dodecyl sulfate 300 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.0; temperature: 303 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
COSYnot availablenot availablenot available
15N T1not availablenot availablenot available
15N T2not availablenot availablenot available
15N Het-NOEnot availablenot availablenot available

Software:

NMRPipe - NMR data processing

X-PLOR v3.581 - structure calculation, structure refinement

Sparky - NMR data analysis

Modelfree - NMR backbone dynamics calculation

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 4432
PDB
DBJ BAA00707 BAC39521 BAG35580
EMBL CAA06711 CAA36258 CAA38987 CAA53468 CAA68378
GB AAB32157 AAB32158 AAG14346 AAG41140 AAG41141
PRF 1309300A
REF NP_000162 NP_001019623 NP_001092519 NP_001139512 NP_001165756
SP O93430 P07727 P23415 P57695 Q5JXX5
TPG DAA13089 DAA27256