BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5614

Title: Sheared A(anti)-A(anti) Pairs in a Destabilizing 2x2 Internal Loop: The NMR Structure of 5'(rGGCAAGCCU)2   PubMed: 12475246

Authors: Znosko, B.; Burkard, M.; Schroeder, S.; Krugh, T.; Turner, D.

Citation: Znosko, B.; Burkard, M.; Schroeder, S.; Krugh, T.; Turner, D.. "Sheared A(anti)*A(anti) base Pairs in a Destabilizing 2 x 2 Internal Loop: The NMR Structure of 5'(rGGCAAGCCU)(2)"  Biochemistry 41, 14969-14977 (2002).

Assembly members:
dlRNA, polymer, 9 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
dlRNA: GGCAAGCCU

Data sets:
Data typeCount
31P chemical shifts8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
15'-R(*GP*GP*CP*AP*AP*GP*CP*CP*U)-31
25'-R(*CP*CP*GP*UP*UP*CP*GP*GP*A)-31

Entities:

Entity 1, 5'-R(*GP*GP*CP*AP*AP*GP*CP*CP*U)-3 9 residues - Formula weight is not available

1   GGCAAGCCU

Samples:

sample_1: dlRNA 1 mM; NaCl 80 mM; phosphate buffer 10 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_2: dlRNA 2 mM; NaCl 80 mM; phosphate buffer 10 mM; EDTA 0.5 mM; D2O 99.996%; D2O 10%

sample_cond_1: ionic strength: 80 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

sample_cond_2: ionic strength: 80 mM; pH: 6.9; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available

Software:

VNMR v5.2 - collection

FELIX v2000 - data analysis

DISCOVER v95.0 - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

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