BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5616

Title: Chemical Shift Assignments of the Prp40 WW Domain Pair   PubMed: 12460579

Authors: Wiesner, Silke; Stier, Gunter; Sattler, Michael; Macias, Maria

Citation: Wiesner, Silke; Stier, Gunter; Sattler, Michael; Macias, Maria. "Solution Structure and Ligand Recognition of the WW Domain Pair of the Yeast Splicing Factor Prp40"  J. Mol. Biol. 324, 807-822 (2002).

Assembly members:
WW domain pair of pre-mRNA processing protein 40, polymer, 86 residues, 10251 Da.

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
WW domain pair of pre-mRNA processing protein 40: MKHHHHHHPMGMSIWKEAKD ASGRIYYYNTLTKKSTWEKP KELISQEELLLRENGWKAAK TADGKVYYYNPTTRETSWTI PAFEKK

Data sets:
Data typeCount
1H chemical shifts517
13C chemical shifts280
15N chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Prp40, WW1-21

Entities:

Entity 1, Prp40, WW1-2 86 residues - 10251 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   GLYMETSERILETRPLYSGLUALALYSASP
3   ALASERGLYARGILETYRTYRTYRASNTHR
4   LEUTHRLYSLYSSERTHRTRPGLULYSPRO
5   LYSGLULEUILESERGLNGLUGLULEULEU
6   LEUARGGLUASNGLYTRPLYSALAALALYS
7   THRALAASPGLYLYSVALTYRTYRTYRASN
8   PROTHRTHRARGGLUTHRSERTRPTHRILE
9   PROALAPHEGLULYSLYS

Samples:

15N-labeled_sample: WW domain pair of pre-mRNA processing protein 40, [U-100% 15N], 1.5 mM

15N_13C_labeled_sample: WW domain pair of pre-mRNA processing protein 40, [U-100% 13C; U-100% 15N], 1.5 mM

Exp_Condition: pH: 6.3; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
HN-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHA-Jnot availablenot availablenot available
HC-HSQCnot availablenot availablenot available
HC(CO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HC(C)H-TOCSYnot availablenot availablenot available
13C-NOESYnot availablenot availablenot available
15N-NOESYnot availablenot availablenot available

Software:

XEASY -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ GAA24712
EMBL CAA81847 CAY81071
GB AAB24902 AHY76231 EDN59896 EDV13075 EDZ70922
REF NP_012913
SP P33203
TPG DAA09144