BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5620

Title: Assignment for ribosomal protein S28E

Authors: Wu, Bin; Yee, Adelinda; Arrowsmith, Cheryl

Citation: Wu, Bin; Yee, Adelinda; Arrowsmith, Cheryl. "Solution structure of ribosomal protein S28E from Methanobacterium Thermoautotrophicum "  The BMRB entry is the only known published source for the data..

Assembly members:
ribosomal protein S28E, polymer, 68 residues, 7666 Da.

Natural source:   Common Name: Methanothermobacter thermoautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ribosomal protein S28E: MDDATPAEVIEVLKRTGMTG EVMQVKCRILDGRDKGRILT RNVMGPIREGDILMLLDTIR EAKEIRTP

Data sets:
Data typeCount
1H chemical shifts483
13C chemical shifts281
15N chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mth02561

Entities:

Entity 1, mth0256 68 residues - 7666 Da.

1   METASPASPALATHRPROALAGLUVALILE
2   GLUVALLEULYSARGTHRGLYMETTHRGLY
3   GLUVALMETGLNVALLYSCYSARGILELEU
4   ASPGLYARGASPLYSGLYARGILELEUTHR
5   ARGASNVALMETGLYPROILEARGGLUGLY
6   ASPILELEUMETLEULEUASPTHRILEARG
7   GLUALALYSGLUILEARGTHRPRO

Samples:

sample_1: ribosomal protein S28E, [U-13C; U-15N], 1 MM; NaCl 450 mM; Na2PO4 25 mM

cond_set1: pH: 6.5 na; temperature: 298 K; ionic strength: 0.45 M

Experiments:

NameSampleSample stateSample conditions
13C-edited NOESY and 15N-edited NOESY were calibrated to obtain accuratesample_1not availablecond_set1
peakposition for structure calculation.sample_1not availablecond_set1

Software:

XEASY - peak assignment

SPARKY v3.95 - peak assignment

NMR spectrometers:

  • VARIAN INOVA 600 MHz

Related Database Links:

PDB
DBJ BAM69468
GB AAB84762 ADL58301
REF NP_275399 WP_010875895 WP_013295525 YP_003849614
SP O26356