BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5621

Title: Assignment for yoag_ecoli

Authors: Wu, Bin; Pineda-Lucena, Antonio; Yee, Adelinda; John, Cort; Kennedy, Michael; Arrowsmith, Cheryl

Citation: Wu, Bin; Pineda-Lucena, Antonio; Yee, Adelinda; John, Cort; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of hypothetical protein dimer encoded by the Yoag gene from Escherichia coli""  The BMRB entry is the only known published source for the data..

Assembly members:
yoag_ecoli, polymer, 60 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
yoag_ecoli: MGKATYTVTVTNNSNGVSVD YETETPMTLLVPEVAAEVIK DLVNTVRSYDTENEHDVCGW

Data sets:
Data typeCount
1H chemical shifts404
13C chemical shifts180
15N chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yoag subunit 11
2yoag subunit 21

Entities:

Entity 1, yoag subunit 1 60 residues - Formula weight is not available

1   METGLYLYSALATHRTYRTHRVALTHRVAL
2   THRASNASNSERASNGLYVALSERVALASP
3   TYRGLUTHRGLUTHRPROMETTHRLEULEU
4   VALPROGLUVALALAALAGLUVALILELYS
5   ASPLEUVALASNTHRVALARGSERTYRASP
6   THRGLUASNGLUHISASPVALCYSGLYTRP

Samples:

sample_1: yoag_ecoli, [U-13C; U-15N], 1.0 mM; NaCl 450 mM; Na2PO4 25 mM

cond_set_1: pH: 6.5 na; temperature: 298 K; ionic strength: 0.45 M

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CC(CO)NH-TOCSYnot availablenot availablenot available
HCC(CO)NH-TOCSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
15N-edited NOESYnot availablenot availablenot available
13C-edited NOESYnot availablenot availablenot available

Software:

XEASY - assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAB35928 BAE76532 BAI55197 BAJ43596 BAL38720
EMBL CAP76288 CAQ32270 CAR03157 CAR08194 CAR13281
GB AAC74866 AAG56785 AAN80661 AAZ88076 ABB61817
REF NP_288232 NP_310532 NP_416310 NP_754096 WP_000513729
SP P64496 P64497 P64498