BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5624

Title: A Conserved Structural Motif at the N-terminal of Bacterial Translation Initiation Factor IF2   PubMed: 12600987

Authors: Laursen, Brian; Mortensen, Kim; Sperling-Petersen, Hans; Hoffman, David

Citation: Laursen, Brian; Mortensen, Kim; Sperling-Petersen, Hans; Hoffman, David. "A Conserved Structural Motif at the N-terminal of Bacterial Translation Initiation Factor IF2"  J. Biol. Chem. 278, 16320-16328 (2003).

Assembly members:
Translation factor IF2, polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Translation factor IF2: TDVTIKTLAAERQTSVERLV QQFADAGIRKSADDSVSAQE KQTLIDHLNQKNSGPDKLTL QRKTRSTLNIPGTGGKSKSV QIEVRKKRTFVKRDPQEAER LAAEEQAQREAEEQARREAE ESAKREAQQKAEREAAEQAK REAAEQAKREAAEKDK

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts753
13C chemical shifts374
15N chemical shifts107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1translation factor IF21

Entities:

Entity 1, translation factor IF2 156 residues - Formula weight is not available

1   THRASPVALTHRILELYSTHRLEUALAALA
2   GLUARGGLNTHRSERVALGLUARGLEUVAL
3   GLNGLNPHEALAASPALAGLYILEARGLYS
4   SERALAASPASPSERVALSERALAGLNGLU
5   LYSGLNTHRLEUILEASPHISLEUASNGLN
6   LYSASNSERGLYPROASPLYSLEUTHRLEU
7   GLNARGLYSTHRARGSERTHRLEUASNILE
8   PROGLYTHRGLYGLYLYSSERLYSSERVAL
9   GLNILEGLUVALARGLYSLYSARGTHRPHE
10   VALLYSARGASPPROGLNGLUALAGLUARG
11   LEUALAALAGLUGLUGLNALAGLNARGGLU
12   ALAGLUGLUGLNALAARGARGGLUALAGLU
13   GLUSERALALYSARGGLUALAGLNGLNLYS
14   ALAGLUARGGLUALAALAGLUGLNALALYS
15   ARGGLUALAALAGLUGLNALALYSARGGLU
16   ALAALAGLULYSASPLYS

Samples:

sample_1: Translation factor IF2 1.5 mM

sample_2: Translation factor IF2, [U-15N], 1.5 mM

sample_3: Translation factor IF2, [U-15N; U-13C], 1.5 mM

sample_4: Translation factor IF2, [U-15N]-Lys, 1.5 mM

conds_1: pH: 6.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HACACBCOnot availablenot availablenot available
15N-edited HSQC TOCSYnot availablenot availablenot available
13C-edited HCCH-TOCSYnot availablenot availablenot available
2QF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

DBJ BAB37472 BAE77214 BAG78978 BAI27448 BAI32627
EMBL CAA05529 CAA05532 CAA05535 CAA05538 CAA05541
GB AAA57971 AAC76202 AAG58304 AAN82365 AAZ89889
PIR D85980
REF NP_289744 NP_312076 NP_417637 NP_755791 WP_000132922
SP A1AG73 A7ZS65 A8A4Y4 B1IQV3 B1LFS0