BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5625

Title: Sequential assignment of the ferricytochrome c3 from Desulfovibrio vulgaris Hildenborough   PubMed: 16527248

Authors: Messias, Ana; Aguiar, Antonio; Turner, David; Xavier, Antonio

Citation: Messiasa, Ana; Aguiara, Antonio; Brennana, Lorraine; Salgueiroa, Carlos; Saraivaa, Ligia; Xaviera, Antonio; Turner, David. "Solution structures of tetrahaem ferricytochrome c3 from Desulfovibrio vulgaris (Hildenborough) and its K45Q mutant: The molecular basis of cooperativity"  Biochim. Biophys. Acta. 1757, 143-153 (2006).

Assembly members:
cytochrome c3, polymer, 107 residues, 14000 Da.
HEC, non-polymer, 618.503 Da.

Natural source:   Common Name: Desulfovibrio vulgaris Hildenborough   Taxonomy ID: 881   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Desulfovibrio vulgaris

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
cytochrome c3: APKAPADGLKMEATKQPVVF NHSTHKSVKCGDCHHPVNGK EDYRKCGTAGCHDSMDKKDK SAKGYYHVMHDKNTKFKSCV GCHVEVAGADAAKKKDLTGC KKSKCHE

Data typeCount
1H chemical shifts742

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c31
2heme, I2
3heme, II2
4heme, III2
5heme, IV2

Entities:

Entity 1, cytochrome c3 107 residues - 14000 Da.

1   ALAPROLYSALAPROALAASPGLYLEULYS
2   METGLUALATHRLYSGLNPROVALVALPHE
3   ASNHISSERTHRHISLYSSERVALLYSCYS
4   GLYASPCYSHISHISPROVALASNGLYLYS
5   GLUASPTYRARGLYSCYSGLYTHRALAGLY
6   CYSHISASPSERMETASPLYSLYSASPLYS
7   SERALALYSGLYTYRTYRHISVALMETHIS
8   ASPLYSASNTHRLYSPHELYSSERCYSVAL
9   GLYCYSHISVALGLUVALALAGLYALAASP
10   ALAALALYSLYSLYSASPLEUTHRGLYCYS
11   LYSLYSSERLYSCYSHISGLU

Entity 2, heme, I - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytochrome c3 2.7 mM; ampicillin 127 uM; kanamicin 78 uM; chloramphenicol 141 uM; H2O 92%; D2O 8%

sample_2: cytochrome c3 2.7 mM; ampicillin 127 uM; kanamicin 78 uM; chloramphenicol 141 uM; D2O 100%

conditions_1: pH*: 7.1; temperature: 303 K

conditions_2: pH*: 7.1; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D-1H-NOESYnot availablenot availablenot available
2D-1H-TOCSYnot availablenot availablenot available
2D-1H-COSYnot availablenot availablenot available

Software:

XEASY -

NMR spectrometers:

  • BRUKER DRX 500 MHz

Related Database Links:

BMRB 4291 5239 6634
PDB
EMBL CAA27847
GB AAS97641 ABM27239 ADP88070
REF WP_010940429 YP_005703708 YP_012381 YP_965666
SP P00131