BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5653

Title: The solution structure of molt-inhibiting hormone from the kuruma prawn Marsupenaeus japonicus

Authors: Katayama, Hidekazu

Citation: Katayama, Hidekazu. "The solution structure of molt-inhibiting hormone from the kuruma prawn Marsupenaeus japonicus"  J. Biol. Chem. ., .-..

Assembly members:
molt-inhibiting hormone, polymer, 78 residues, 9134 Da.

Natural source:   Common Name: kuruma prawn   Taxonomy ID: 27405   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Marsupenaeus japonicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
molt-inhibiting hormone: ASFIDNTCRGVMGNRDIYKK VVRVCEDCTNIFRLPGLDGM CRNRCFYNEWFLICLKAANR EDEIEKFRVWISILNAGQ

Data sets:
Data typeCount
1H chemical shifts528
13C chemical shifts343
15N chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MIH monomer1

Entities:

Entity 1, MIH monomer 78 residues - 9134 Da.

1   ALASERPHEILEASPASNTHRCYSARGGLY
2   VALMETGLYASNARGASPILETYRLYSLYS
3   VALVALARGVALCYSGLUASPCYSTHRASN
4   ILEPHEARGLEUPROGLYLEUASPGLYMET
5   CYSARGASNARGCYSPHETYRASNGLUTRP
6   PHELEUILECYSLEULYSALAALAASNARG
7   GLUASPGLUILEGLULYSPHEARGVALTRP
8   ILESERILELEUASNALAGLYGLN

Samples:

sample_1: molt-inhibiting hormone, [U-15N; U-13C], 1.5 – 2.0 mM

sample_2: molt-inhibiting hormone, [U-15N], 1.5 – 2.0 mM

Ex-cond_1: pH: 7.4; temperature: 298 K; ionic strength: 0 M

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
(HCA)CO(CA)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
13C-edited NOESYnot availablenot availablenot available
15N-edited NOESYnot availablenot availablenot available

Software:

nmrPipe v1.7 -

SPARKY v3.76 - peak picking

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

DBJ BAA20432
SWISS-PROT P55847
BMRB 5633
PDB