BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5664

Title: Full 1H and 15N Chemical Shift Assignments for Oxytetracycline Acyl Carrier Protein   PubMed: 12859187

Authors: Findlow, Stuart; Winsor, Claire; Dempsey, Christopher; Crosby, John; Simpson, Thomas; Crump, Matthew

Citation: Findlow, Stuart; Winsor, Claire; Simpson, Thomas; Crosby, John; Crump, Matthew. "Solution Structure and Dynamics of Oxytetracycline Polyketide Synthase Acyl Carrier Protein from Streptomyces rimosus "  Biochemistry 42, 8423-8433 (2003).

Assembly members:
Acyl Carrier Protein, polymer, 95 residues, 9916 Da.

Natural source:   Common Name: soil bacterium   Taxonomy ID: 1927   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces rimosus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Acyl Carrier Protein: MTLLTLSDLLTLLRECAGEE ESIDLGGDVEDVAFDALGYD SLALLNTVGRIERDYGVQLG DDAVEKATTPRALIEMTNAS LTGASPSAGGAARDK

Data sets:
Data typeCount
1H chemical shifts585
15N chemical shifts95

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1otc ACP1

Entities:

Entity 1, otc ACP 95 residues - 9916 Da.

1   METTHRLEULEUTHRLEUSERASPLEULEU
2   THRLEULEUARGGLUCYSALAGLYGLUGLU
3   GLUSERILEASPLEUGLYGLYASPVALGLU
4   ASPVALALAPHEASPALALEUGLYTYRASP
5   SERLEUALALEULEUASNTHRVALGLYARG
6   ILEGLUARGASPTYRGLYVALGLNLEUGLY
7   ASPASPALAVALGLULYSALATHRTHRPRO
8   ARGALALEUILEGLUMETTHRASNALASER
9   LEUTHRGLYALASERPROSERALAGLYGLY
10   ALAALAARGASPLYS

Samples:

sample_1: Acyl Carrier Protein, [U-97% 15N], 1 mM

Ex-cond_1: pH: 5.3 na; temperature: 298 K; ionic strength: 0.015 M

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

NMRPipe - NMR data processing

Pipp - NMR data assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA80986
GB AAA03334 AAZ78327 ELQ83294 KEF12048
REF WP_003981021 WP_030372734
SP P43677