BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5669

Title: Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4   PubMed: 12644454

Authors: Wang, B.; Alam, S.; Meyer, H.; Payne, M.; Stemmler, T.; Davis, D.; Sundquist, W.

Citation: Wang, B.; Alam, S.; Meyer, H.; Payne, M.; Stemmler, T.; Davis, D.; Sundquist, W.. "Structure and Ubiquitin Interactions of the Conserved Zinc Finger Domain of Npl4"  J. Biol. Chem. 278, 20225-20234 (2003).

Assembly members:
Npl4 Zinc Finger, polymer, 31 residues, 3373 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Npl4 Zinc Finger: GSTSAMWACQHCTFMNQPGT GHCEMCSLPRT

Data sets:
Data typeCount
13C chemical shifts109
15N chemical shifts30
1H chemical shifts164
coupling constants13

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Npl4 Zinc Finger domain1
2ZINC ION2

Entities:

Entity 1, Npl4 Zinc Finger domain 31 residues - 3373 Da.

1   GLYSERTHRSERALAMETTRPALACYSGLN
2   HISCYSTHRPHEMETASNGLNPROGLYTHR
3   GLYHISCYSGLUMETCYSSERLEUPROARG
4   THR

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Npl4 Zinc Finger, [U-15N; U-13C], 1 mM; sodium phosphate 20 mM; NaCl 50 mM; BME 5 mM; H2O 90%; D2O 10%

sample_2: Npl4 Zinc Finger, [U-95% 15N], 1 mM

sample_cond_1: ionic strength: 75 mM; pH: 5.5; pressure: 1 atm; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablesample_cond_1
HN(CA)COnot availablenot availablesample_cond_1
H(CCO)NH-TOCSYnot availablenot availablesample_cond_1
(H)C(CO)NH-TOCSYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1

Software:

CNS v1.1 - refinement

DYANA v1.5 - refinement

FELIX v97 - processing

SPARKY v3.106 - data analysis

NMR spectrometers:

  • Varian UNITYplus 500 MHz
  • Varian Inova 600 MHz

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