BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5671

Title: Overall structure and sugar dynamics of a DNA dodecamer from homo and heteronuclear dipolar couplings and 31P chemical shift anisotropy   PubMed: 12815257

Authors: Zhengrong, W.; Delaglio, F.; Tjandra, N.; Zhurkin, V.; Bax, A.

Citation: Wu, Z.; Delaglio, F.; Tjandra, N.; Zhurkin, V.; Bax, A.. "Overall Structure and Sugar Dynamics of a DNA Dodecamer from Homo- and Heteronuclear Dipolar Couplings and 31P Chemical Shift Anisotropy"  J. Biomol. NMR 26, 297-315 (2003).

Assembly members:
Dodecamer, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Dodecamer: CGCGAATTCGCG

Data typeCount
31P chemical shifts22
coupling constants22
residual dipolar couplings636

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dodecamer, chain A1
2Dodecamer, chain B1

Entities:

Entity 1, Dodecamer, chain A 12 residues - Formula weight is not available

1   DCDGDCDGDADADTDTDCDG
2   DCDG

Samples:

sample_1: Dodecamer 0.5 mM; sodium phosphate 40 mM; NaN3 0.04%; DMPC/DHPC/CTAB (30/10/1) 5%; D2O 100%

sample_2: Dodecamer, [U-13C; U-15N], 0.5 mM; sodium phosphate 40 mM; NaN3 0.04%; pf1 phage 20 mg/mL; D2O 10%; H2O 90%

sample_cond_1: ionic strength: 40 mM; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
H-31P-HSQCnot availablenot availablesample_cond_1
HP-ctNOESY-DIF COSYnot availablenot availablesample_cond_1
COSYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
13C-HSQC-J-MODULATEDnot availablenot availablesample_cond_1
3C-HSQC-F1-COUPLEDnot availablenot availablesample_cond_1
15N-HSQC-F1-COUPLEDnot availablenot availablesample_cond_1

Software:

NMRPipe v1.7 REV 1999.039.11.31 - data analysis

xwinnmr v2.4 and 2.6 - collection

X-PLOR v3.841 - refinement

CosyView - data analysis

NMR spectrometers:

  • Bruker AVANCE 800 MHz

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