BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5673

Title: NMR Solution Structure of the Glucagon Antagonist [desHis1, desPhe6, Glu9]Glucagon Amide in the Presence of Perdeuterated Dodecylphosphocholine Micelles   PubMed: 12627948

Authors: Ying, J.; Ahn, J.-M.; Jacobsen, N.; Brown, M.; Hruby, V.

Citation: Ying, J.; Ahn, J.-M.; Jacobsen, N.; Brown, M.; Hruby, V.. "NMR Solution Structure of the Glucagon Antagonist [desHis(1), desPhe(6), Glu(9)] Glucagon Amide in the Presence of Perdeuterated Dodecylphosphocholine Micelles"  Biochemistry 42, 2825-2835 (2003).

Assembly members:
Glucagon analogue, polymer, 28 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Glucagon analogue: SQGTTSEYSKYLDSRRAQDF VQWLMNTX

Data sets:
Data typeCount
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Glucagon analogue1

Entities:

Entity 1, Glucagon analogue 28 residues - Formula weight is not available

1   SERGLNGLYTHRTHRSERGLUTYRSERLYS
2   TYRLEUASPSERARGARGALAGLNASPPHE
3   VALGLNTRPLEUMETASNTHRNH2

Samples:

sample_1: Glucagon analogue 7 mM; sodium phosphate buffer 50 mM; dodecylphosphocholine-d38 283 mM; sodium azide 1 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1

Software:

UXNMR - collection

FELIX v2000 - data analysis, processing

DGII vwithin InsightII 2000 - structure solution

DISCOVER vwithin InsightII 2000 - refinement

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB