BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5689

Title: 1H, 13C, and 15N backbone assignments of the pheromone binding protein from the silk moth Antheraea polyphemus (ApolPBP)   PubMed: 14512737

Authors: Mohanty, Smita; Zubkov, Sergey; Campos-Olivas, Ramon

Citation: Mohanty, Smita; Zubkov, Sergey; Campos-Olivas, Ramon. "Letter to the Editor: 1H, 13C and 15N Backbone Assignments of the Pheromone Binding Protein from the Silk Moth Antheraea polyphemus (ApolPBP) "  J. Biomol. NMR 27, 393-394 (2003).

Assembly members:
Pheromone-binding protein from Antheraea polyphemus, polymer, 142 residues, Formula weight is not available

Natural source:   Common Name: Polyphemus moth   Taxonomy ID: 7120   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Antheraea polyphemus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Pheromone-binding protein from Antheraea polyphemus: SPEIMKNLSNNFGKAMDQCK DELSLPDSVVADLYNFWKDD YVMTDRLAGCAINCLATKLD VVDPDGNLHHGNAKDFAMKH GADETMAQQLVDIIHGCEKS APPNDDKCMKTIDVAMCFKK EIHKLNWVPNMDLVIGEVLA EV

Data sets:
Data typeCount
1H chemical shifts939
13C chemical shifts548
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ApolPBP1

Entities:

Entity 1, ApolPBP 142 residues - Formula weight is not available

1   SERPROGLUILEMETLYSASNLEUSERASN
2   ASNPHEGLYLYSALAMETASPGLNCYSLYS
3   ASPGLULEUSERLEUPROASPSERVALVAL
4   ALAASPLEUTYRASNPHETRPLYSASPASP
5   TYRVALMETTHRASPARGLEUALAGLYCYS
6   ALAILEASNCYSLEUALATHRLYSLEUASP
7   VALVALASPPROASPGLYASNLEUHISHIS
8   GLYASNALALYSASPPHEALAMETLYSHIS
9   GLYALAASPGLUTHRMETALAGLNGLNLEU
10   VALASPILEILEHISGLYCYSGLULYSSER
11   ALAPROPROASNASPASPLYSCYSMETLYS
12   THRILEASPVALALAMETCYSPHELYSLYS
13   GLUILEHISLYSLEUASNTRPVALPROASN
14   METASPLEUVALILEGLYGLUVALLEUALA
15   GLUVAL

Samples:

sample_1: Pheromone-binding protein from Antheraea polyphemus, [U-99% 13C; U-99% 15N], 1.0 mM

backbone_conditions: pH: 6.33; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HMQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D CC(CO)NHnot availablenot availablenot available
3D HCACOnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D 1H-15N-1H NOESYnot availablenot availablenot available
3D 1H-13C-1H NOESYnot availablenot availablenot available

Software:

NMRPipe v2.1 -

NMRView v5.0.4 -

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15256
PDB
EMBL CAA35592
GB AAB49502
PRF 1515253A
SP P20797