BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5695

Title: Solution Structure of the Product of the S. Cerevisiae YHR087W Gene, Northeast Structural Genomics Consortium target YTYst425

Authors: Cort, John; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michael

Citation: Cort, John; Yee, Adelinda; Arrowsmith, Cheryl; Kennedy, Michael. "Solution Structure of the Product of the S. Cerevisiae YHR087W Gene"  The BMRB entry is the only known published source for the data..

Assembly members:
YHR087W, polymer, 131 residues, 12000 Da.

Natural source:   Common Name: Yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
YHR087W: MGSSHHHHHHSSGLVPRGSH MSTVTKYFYKGENTDLIVFA ASEELVDEYLKNPSIGKLSE VVELFEVFTPQDGRGAEGEL GAASKAQVENEFGKGKKIEE VIDLILRNGKPNSTTSSLKT KGGNAGTKAYN

Data sets:
Data typeCount
1H chemical shifts547
13C chemical shifts355
15N chemical shifts78
coupling constants60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YHRO87W1

Entities:

Entity 1, YHRO87W 131 residues - 12000 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERTHRVALTHRLYSTYRPHETYRLYS
4   GLYGLUASNTHRASPLEUILEVALPHEALA
5   ALASERGLUGLULEUVALASPGLUTYRLEU
6   LYSASNPROSERILEGLYLYSLEUSERGLU
7   VALVALGLULEUPHEGLUVALPHETHRPRO
8   GLNASPGLYARGGLYALAGLUGLYGLULEU
9   GLYALAALASERLYSALAGLNVALGLUASN
10   GLUPHEGLYLYSGLYLYSLYSILEGLUGLU
11   VALILEASPLEUILELEUARGASNGLYLYS
12   PROASNSERTHRTHRSERSERLEULYSTHR
13   LYSGLYGLYASNALAGLYTHRLYSALATYR
14   ASN

Samples:

sample_1: YHR087W, [U-13C; U-15N], 1 mM; sodium acetate 10 mM; sodium chloride 300 mM

sample_2: YHR087W, [U-10% 13C; 99%-15N], 1 mM; sodium acetate 10 mM; sodium chloride 300 mM

Ex-cond_1: pH: 5.0; temperature: 298 K; ionic strength: 0.31 M

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
HCC-TOCSY-NNHnot availablenot availableEx-cond_1
CCC-TOCSY-NNHnot availablenot availableEx-cond_1
CBCACOCAHAnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 750 MHz
  • Varian Inova 600 MHz
  • Varian Unity 600 MHz

Related Database Links:

PDB
DBJ GAA23805
EMBL CAY80098
GB AAB68927 AAS56171 AHY77788 EDN62328 EDV09136
REF NP_011955
SP P38804
TPG DAA06783