BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5696

Title: 1H, 15N and 13C resonance assignments of the PDZ domain of ZASP   PubMed: 15062084

Authors: Au, Yunghan; Atkinson, R.; Faulkner, Georgine; Frenkiel, Thomas; Joseph, Catherine; Kelly, Geoff; Muskett, Frederick; Pallavicini, Alberto; Pastore, Annalisa

Citation: Au, Yunghan; Atkinson, R.; Guerrini, R.; Kelly, Geoff; Joseph, Catherine; Martin, S.; Muskett, Frederick; Pallavicini, Alberto; Faulkner, Georgine; Pastore, Annalisa. "Solution Structure of ZASP PDZ Domain; Implications for Sarcomere Ultrastructure and Enigma Family Redundancy"  Structure (Cambridge, MA, U. S.) 12, 611-622 (2004).

Assembly members:
Z-band alternatively spliced PDZ motif protein, polymer, 85 residues, 9139.40 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Z-band alternatively spliced PDZ motif protein: MAYSVTLTGPGPWGFRLQGG KDFNMPLTISRITPGSKAAQ SQLSQGDLVVAIDGVNTDTM THLEAQNKIKSASYNLSLTL QKSKR

Data sets:
Data typeCount
1H chemical shifts577
13C chemical shifts335
15N chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ZASP-PDZ Domain1

Entities:

Entity 1, ZASP-PDZ Domain 85 residues - 9139.40 Da.

1   METALATYRSERVALTHRLEUTHRGLYPRO
2   GLYPROTRPGLYPHEARGLEUGLNGLYGLY
3   LYSASPPHEASNMETPROLEUTHRILESER
4   ARGILETHRPROGLYSERLYSALAALAGLN
5   SERGLNLEUSERGLNGLYASPLEUVALVAL
6   ALAILEASPGLYVALASNTHRASPTHRMET
7   THRHISLEUGLUALAGLNASNLYSILELYS
8   SERALASERTYRASNLEUSERLEUTHRLEU
9   GLNLYSSERLYSARG

Samples:

Sample_1: Z-band alternatively spliced PDZ motif protein, [U-15N; U-13C], 0.5 – 1.5 mM; sodium phosphate 20 mM; sodium azide 0.2 % v/v; deuterium oxide 10 % v/v

Sample_2: Z-band alternatively spliced PDZ motif protein, [U-15N], 0.5 – 1.5 mM

Condition_1: pH: 6.6; temperature: 300 K; ionic strength: 0.02 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

nmrPipe - processing

XEASY - analysis

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian UnityPlus 600 MHz
  • Varian UnityPlus 800 MHz

Related Database Links:

BMRB 10061
PDB
DBJ BAA31588 BAB23128 BAD32258 BAE23262 BAE25016
EMBL CAB46727 CAB46728 CAB46729 CAB46747
GB AAD42950 AAD42951 AAF33847 AAF33848 AAH10929
REF NP_001030493 NP_001034160 NP_001034161 NP_001034162 NP_001034163
SP O75112 Q9JKS4
TPG DAA14160