BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5706

Title: Antibiotic binding domain of a TipA-class multidrug resistance transcriptional regulator   PubMed: 12682015

Authors: Kahmann, Jan; Sass, Hans Juergen; Allan, Martin; Seto, Haruo; Thompson, Charles; Grzesiek, Stephan

Citation: Kahmann, Jan; Sass, Hans Juergen; Allan, Martin; Seto, Haruo; Thompson, Charles; Grzesiek, Stephan. "Structural Basis for Antibiotic Recognition by the TipA Class of Multidrug-resistance Transcriptional Regulators"  EMBO J. 22, 1824-1834 (2003).

Assembly members:
Transcriptional activator tipA, polymer, 143 residues, 16432 Da.

Natural source:   Common Name: Streptomyces lividans   Taxonomy ID: 1916   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces lividans

Experimental source:   Production method: recombinant tecHology   Host organism: Escherichia lividans

Entity Sequences (FASTA):
Transcriptional activator tipA: GINLTPEEKFEVFGDFDPDQ YEEEVRERWGNTDAYRQSKE KTASYTKEDWQRIQDEADEL TRRFVALMDAGEPADSEGAM DAAEDHRQGIARNHYDCGYE MHTCLGEMYVSDERFTRNID AAKPGLAAYMRDAILANAVR HTP

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts473
13C chemical shifts345
15N chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TipAS1

Entities:

Entity 1, TipAS 143 residues - 16432 Da.

1   GLYILEASNLEUTHRPROGLUGLULYSPHE
2   GLUVALPHEGLYASPPHEASPPROASPGLN
3   TYRGLUGLUGLUVALARGGLUARGTRPGLY
4   ASNTHRASPALATYRARGGLNSERLYSGLU
5   LYSTHRALASERTYRTHRLYSGLUASPTRP
6   GLNARGILEGLNASPGLUALAASPGLULEU
7   THRARGARGPHEVALALALEUMETASPALA
8   GLYGLUPROALAASPSERGLUGLYALAMET
9   ASPALAALAGLUASPHISARGGLNGLYILE
10   ALAARGASNHISTYRASPCYSGLYTYRGLU
11   METHISTHRCYSLEUGLYGLUMETTYRVAL
12   SERASPGLUARGPHETHRARGASNILEASP
13   ALAALALYSPROGLYLEUALAALATYRMET
14   ARGASPALAILELEUALAASNALAVALARG
15   HISTHRPRO

Samples:

sample_1: Transcriptional activator tipA, [U-15N], 1.5 mM

sample_2: Transcriptional activator tipA, [U-13C; U-15N], 1.5 mM

Ex-cond_1: pH: 5.9 na; temperature: 298 K; ionic strength: 0.006 M

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available

Software:

NMRPipe v2.1 - processing

Pipp v4.3.2 - data analysis

Xeasy v1.2 - data analysis

NMR spectrometers:

  • DRX Bruker 600 MHz
  • DRX Bruker 800 MHz

Related Database Links:

BMRB 19421 19422
PDB
DBJ BAD11210 BAD11216 BAD11222 BAD11228 BAD11234
EMBL CAB42766
GB AAB27737 AAC13653 AIJ15084 EFD68501 EHN72093
REF NP_627619 WP_003975420
SP P0A4T8 P0A4T9