BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5708

Title: Solution structure of the N-terminal membrane anchor of E. coli enzyme IIA(Glucose)   PubMed: 12717030

Authors: Wang, G.; Keifer, P.; Peterkofsky, A.

Citation: Wang, G.; Keifer, P.; Peterkofsky, A.. "Solution Structure of the N-terminal Amphitropic Domain of Escherichia coli Glucose-specific Enzyme IIA in Membrane-mimetic Micelles"  Protein Sci. 12, 1087-1096 (2003).

Assembly members:
PTS, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PTS: GLFDKLKSLVSDDKK

Data sets:
Data typeCount
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PTS system, glucose-specific IIA component1

Entities:

Entity 1, PTS system, glucose-specific IIA component 15 residues - Formula weight is not available

1   GLYLEUPHEASPLYSLEULYSSERLEUVAL
2   SERASPASPLYSLYS

Samples:

sample_1: PTS 5 mM; H2O 90%; D2O 10%; SDS 100 mM

sample_2: PTS 5 mM; DHPG 50 mM

sample_cond_1: pH: 5.4; temperature: 298 K; ionic strength: 0 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

NMRPipe v2.1 - data processing

nmrDraw v2.1 - data processing

PIPP v1.0 - NOE picking

XPLOR-NIH v1.06 - structural calculations

MOLMOL v2K.1 - structural analysis, structural viewing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

EMBL CDL35253
GB EHV05336 EKP27219
REF WP_004870785