BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5710

Title: Structure and interactions of Mth11: A homologue of human RNase P protein Rpp29   PubMed: 14673079

Authors: Boomershine, William; McElroy, Craig; Tsai, Hsin-Yue; Gopalan, Venkat; Foster, Mark

Citation: Boomershine, William; McElroy, Craig; Tsai, Hsin-Yue; Wilson, R; Gopalan, Venkat; Foster, Mark. " Structure of Mth11/Mth Rpp29, an essential protein subunit of archaeal and eukaryotic RNase P"  Proc. Natl. Acad. Sci. U. S. A. 100, 15398-15403 (2003).

Assembly members:
Mth11, polymer, 97 residues, Formula weight is not available

Natural source:   Common Name: Methanothermobacter thermautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanothermobacter thermoautotrophicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Mth11: GSHMLITPRNIFRHELIGLS VRIARSVHRDIQGISGRVVD ETRNTLRIEMDDGREITVPK GIAVFHFRTPQGELVEIDGR ALVARPEERIKKKFRKP

Data sets:
Data typeCount
1H chemical shifts581
13C chemical shifts288
15N chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mth11 monomer1

Entities:

Entity 1, mth11 monomer 97 residues - Formula weight is not available

1   GLYSERHISMETLEUILETHRPROARGASN
2   ILEPHEARGHISGLULEUILEGLYLEUSER
3   VALARGILEALAARGSERVALHISARGASP
4   ILEGLNGLYILESERGLYARGVALVALASP
5   GLUTHRARGASNTHRLEUARGILEGLUMET
6   ASPASPGLYARGGLUILETHRVALPROLYS
7   GLYILEALAVALPHEHISPHEARGTHRPRO
8   GLNGLYGLULEUVALGLUILEASPGLYARG
9   ALALEUVALALAARGPROGLUGLUARGILE
10   LYSLYSLYSPHEARGLYSPRO

Samples:

sample_1: Mth11, [U-98% 13C; U-98% 15N], 1.0 – 1.5 mM

Ex-cond_1: pH: 7.0; temperature: 299 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
GB AAB84512
REF NP_275156 WP_010875653
SP O26119