BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5722

Title: Chemical Shift Assignmens of Ca2+/C-terminal Domain of Caltractin (1H, 13C, and 15N) in Complex with the Cdc31p-Binding Domain from Kar1p (1H)   PubMed: 12842464

Authors: Hu, Haitao; Chazin, Walter

Citation: Hu, Haitao; Chazin, Walter. "Unique Features in the C-terminal Domain Provide Caltractin with Target Specificity"  J. Mol. Biol. 330, 473-484 (2003).

Assembly members:
C-terminal domain of caltractin, polymer, 77 residues, 8966 Da.
19-residue cdc31p-binding domain from Kar1p, polymer, 19 residues, 2492 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: green algae   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Chlamydomonas reinhardtii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
C-terminal domain of caltractin: GSGERDSREEILKAFRLFDD DNSGTITIKDLRRVAKELGE NLTEEELQEMIAEADRNDDN EIDEDEFIRIMKKTSLF
19-residue cdc31p-binding domain from Kar1p: KKRELIESKWHRLLFHDKK

Data sets:
Data typeCount
1H chemical shifts686
13C chemical shifts317
15N chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain of caltractin1
219-residue Kar1p peptide2
3CALCIUM (II) ION3

Entities:

Entity 1, C-terminal domain of caltractin 77 residues - 8966 Da.

1   GLYSERGLYGLUARGASPSERARGGLUGLU
2   ILELEULYSALAPHEARGLEUPHEASPASP
3   ASPASNSERGLYTHRILETHRILELYSASP
4   LEUARGARGVALALALYSGLULEUGLYGLU
5   ASNLEUTHRGLUGLUGLULEUGLNGLUMET
6   ILEALAGLUALAASPARGASNASPASPASN
7   GLUILEASPGLUASPGLUPHEILEARGILE
8   METLYSLYSTHRSERLEUPHE

Entity 2, 19-residue Kar1p peptide 19 residues - 2492 Da.

1   LYSLYSARGGLULEUILEGLUSERLYSTRP
2   HISARGLEULEUPHEHISASPLYSLYS

Entity 3, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: C-terminal domain of caltractin, [U-13C; U-15N], 1.0 mM; 19-residue cdc31p-binding domain from Kar1p 1.2 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ GAA25914
EMBL CAA96082 CAY82415 CAA96082
GB AAA34716 AAT92901 AHY76913 EDN62631 EDV12432
REF NP_014211 NP_014211
SP P11927
TPG DAA10365
SWISS-PROT P11927
GenBank EDN62631 EDV12432 AAA34716 AAT92901