BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5729

Title: Solution Structure, Dynamics and Thermodynamics of the Native State Ensemble of the Sem-5 C-terminal SH3 Domain   PubMed: 12741814

Authors: Chu, Josephine; Volk, David; Luxon, Bruce; Gorenstein, David; Hilser, Vincent

Citation: Ferreon, Josephine; Volk, David; Luxon, Bruce; Gorenstein, David; Hilser, Vincent. "Solution Structure, Dynamics, and Thermodynamics of the Native State Ensemble of the Sem-5 C-terminal SH3 Domain"  Biochemistry 42, 5582-5591 (2003).

Assembly members:
SH3 Domain, polymer, 62 residues, Formula weight is not available

Natural source:   Common Name: 6239   Taxonomy ID: C. elegans   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
SH3 Domain: HMETKFVEALFDFNPQESGE LAFKRGDVITLINKDDPNWW EGQLNNRRGIFPSNYVAPYN SN

Data sets:
Data typeCount
13C chemical shifts293
15N chemical shifts68
1H chemical shifts439

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 subunit 1, monomer1

Entities:

Entity 1, SH3 subunit 1, monomer 62 residues - Formula weight is not available

1   HISMETGLUTHRLYSPHEVALGLUALALEU
2   PHEASPPHEASNPROGLNGLUSERGLYGLU
3   LEUALAPHELYSARGGLYASPVALILETHR
4   LEUILEASNLYSASPASPPROASNTRPTRP
5   GLUGLYGLNLEUASNASNARGARGGLYILE
6   PHEPROSERASNTYRVALALAPROTYRASN
7   SERASN

Samples:

sample_1: SH3 Domain, [U-95% 13C; U-95% 15N], 2.0 mM

Ex-cond_1: pH: 4.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian UnityPlus 400 MHz
  • Varian UnityPlus 750 MHz

Related Database Links:

BMRB 16462
PDB