BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5738

Title: The NMR Structure of a Bifunctional Rhodamine Labeled N-domain of Troponin C in Complex with the Regulatory 'Switch' Peptide from Troponin I   PubMed: 12693929

Authors: Mercier, P.; Ferguson, R.; Irving, M.; Corrie, J.; Trentham, D.; Sykes, B.

Citation: Mercier, P.; Ferguson, R.; Irving, M.; Corrie, J.; Trentham, D.; Sykes, B.. "NMR Structure of a Bifunctional Rhodamine Labeled N-domain of Troponin C Complexed with the Regulatory 'Switch' Peptide from Troponin I: Implications for in situ Fluorescence Studies in Muscle Fibers "  Biochemistry 42, 4333-4348 (2003).

Assembly members:
Troponin C, polymer, 90 residues, Formula weight is not available
Troponin I, polymer, 17 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Troponin C: ASMTDQQAEARAFLSEEMIA EFKAAFDMFDADGGGDISTK ELGTVMRMLGQNPTKCELDA IICEVDEDGSGTIDFEEFLV MMVRQMKEDA
Troponin I: RMSADAMLRALLGSKHK

Data sets:
Data typeCount
1H chemical shifts629
13C chemical shifts263
15N chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Troponin C1
2Troponin I2
3CALCIUM ION(II) 13
4CALCIUM ION(II) 23

Entities:

Entity 1, Troponin C 90 residues - Formula weight is not available

1   ALASERMETTHRASPGLNGLNALAGLUALA
2   ARGALAPHELEUSERGLUGLUMETILEALA
3   GLUPHELYSALAALAPHEASPMETPHEASP
4   ALAASPGLYGLYGLYASPILESERTHRLYS
5   GLULEUGLYTHRVALMETARGMETLEUGLY
6   GLNASNPROTHRLYSCYSGLULEUASPALA
7   ILEILECYSGLUVALASPGLUASPGLYSER
8   GLYTHRILEASPPHEGLUGLUPHELEUVAL
9   METMETVALARGGLNMETLYSGLUASPALA

Entity 2, Troponin I 17 residues - Formula weight is not available

1   ARGMETSERALAASPALAMETLEUARGALA
2   LEULEUGLYSERLYSHISLYS

Entity 3, CALCIUM ION(II) 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Troponin C 1 mM; Troponin I 1 mM; Ca2+ 2 mM; KCl 320 mM; imidazole 10 mM; NaN3 3%; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5; temperature: 303 K; ionic strength: 320 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablesample_cond_1
3D 15N/13C-NOESYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1
2D 13C/15N-edited-NOESYsample_1not availablesample_cond_1
2D 13C/15N-filtered/edited-NOESYsample_1not availablesample_cond_1

Software:

CNS v1.1 - structure solution, refinement

NMRPipe vFeb 2002 - processing

nmrview v5.0.4 - data analysis

Procheck v3.5.4 - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 16414
PDB
EMBL CAA27447 CAA27447
GB AAA61952 AAB00122 AAH44282 AAH84508 AAL86905
REF NP_001011110 NP_001079556 NP_990748 XP_001518819 XP_002199061 XP_001518819 NP_990748 NP_001079556 NP_001011110
SP P68246 P68247
SWISS-PROT P68247 P68246
GenBank AAL86905 AAH84508 AAH44282 AAB00122 AAA61952