BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5742

Title: Solution structure of soluble domain of Sco1 from Bacillus Subtilis   PubMed: 14604533

Authors: Balatri, E.; Banci, L.; Bertini, I.; Cantini, F.; Ciofi-Baffoni, S.

Citation: Balatri, Erica; Banci, Lucia; Bertini, Ivano; Cantini, Francesca; Ciofi-Baffoni, Simone. "Solution structure of Sco1: a thioredoxin-like protein involved in cytochrome c oxidase assembly"  Structure (Cambridge, MA, U. S.) 11, 1431-1443 (2003).

Assembly members:
Sco1, polymer, 174 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Sco1: HMLEIKDPLNYEVEPFTFQN QDGKNVSLESLKGEVWLADF IFTNCETICPPMTAHMTDLQ KKLKAENIDVRIISFSVDPE NDKPKQLKKFAANYPLSFDN WDFLTGYSQSEIEEFALKSF KAIVKKPEGEDQVIHQSSFY LVGPDGKVLKDYNGVENTPY DDIISDVKSASTLK

Data sets:
Data typeCount
1H chemical shifts1095
13C chemical shifts398
15N chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sco11

Entities:

Entity 1, Sco1 174 residues - Formula weight is not available

1   HISMETLEUGLUILELYSASPPROLEUASN
2   TYRGLUVALGLUPROPHETHRPHEGLNASN
3   GLNASPGLYLYSASNVALSERLEUGLUSER
4   LEULYSGLYGLUVALTRPLEUALAASPPHE
5   ILEPHETHRASNCYSGLUTHRILECYSPRO
6   PROMETTHRALAHISMETTHRASPLEUGLN
7   LYSLYSLEULYSALAGLUASNILEASPVAL
8   ARGILEILESERPHESERVALASPPROGLU
9   ASNASPLYSPROLYSGLNLEULYSLYSPHE
10   ALAALAASNTYRPROLEUSERPHEASPASN
11   TRPASPPHELEUTHRGLYTYRSERGLNSER
12   GLUILEGLUGLUPHEALALEULYSSERPHE
13   LYSALAILEVALLYSLYSPROGLUGLYGLU
14   ASPGLNVALILEHISGLNSERSERPHETYR
15   LEUVALGLYPROASPGLYLYSVALLEULYS
16   ASPTYRASNGLYVALGLUASNTHRPROTYR
17   ASPASPILEILESERASPVALLYSSERALA
18   SERTHRLEULYS

Samples:

sample_1: Sco1, [U-15N; U-13C], 1.5 mM; potassium phosphate 100 mM; DTT 2 mM; D2O 10%

sample_2: Sco1 1.5 mM; potassium phosphate 100 mM; DTT 2 mM; D2O 90%

sample_cond_1: pH: 7 na; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
(H)CCH-TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

XWINNMR - collection, processing

XEASY vversion 1.3 - data analysis

GARANT v2.0 - data analysis

DYANA v1.5 - structure solution

CYANA v1.0 - structure solution

AMBER v5 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz
  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 700 MHz
  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAM52657 BAM58232
EMBL CAB14093 CCU58674 CEI57387 CEJ77812
GB AAA96641 ADV92901 AEP91191 AFQ58121 AGA23479
REF NP_390058 WP_003230805 WP_003245947 WP_014477121 WP_014906386
SP P54178