BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5745

Title: NMR Structure of bovine cytochrome b5   PubMed: 8613986

Authors: Muskett, Frederick; Whitford, David

Citation: Muskett, Frederick; Whitford, David. "The solution structure of bovine ferricytochrome b5 determined using heteronuclear NMR methods"  J. Biomol. NMR 258, 172-189 (1996).

Assembly members:
Cytochrome b5, polymer, 104 residues, 11795 Da.
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cytochrome b5: AEESSKAVKYYTLEEIQKHN NSKSTWLILHYKVYDLTKFL EEHPGGEEVLREQAGGDATE NFEDVGHSTDARELSKTFII GELHPDDRSKITKPSESIIT TIDS

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts508
13C chemical shifts307
15N chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome b5 monomer1
2HEM_ox2

Entities:

Entity 1, cytochrome b5 monomer 104 residues - 11795 Da.

1   ALAGLUGLUSERSERLYSALAVALLYSTYR
2   TYRTHRLEUGLUGLUILEGLNLYSHISASN
3   ASNSERLYSSERTHRTRPLEUILELEUHIS
4   TYRLYSVALTYRASPLEUTHRLYSPHELEU
5   GLUGLUHISPROGLYGLYGLUGLUVALLEU
6   ARGGLUGLNALAGLYGLYASPALATHRGLU
7   ASNPHEGLUASPVALGLYHISSERTHRASP
8   ALAARGGLULEUSERLYSTHRPHEILEILE
9   GLYGLULEUHISPROASPASPARGSERLYS
10   ILETHRLYSPROSERGLUSERILEILETHR
11   THRILEASPSER

Entity 2, HEM_ox - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Cytochrome b5, [U-13C; U-15N], 1.0 – 2.0 mM; Phosphate 20 mM

Experimental_Conditions: pH: 7.0; temperature: 300 K; ionic strength: 0.08 M

Experiments:

NameSampleSample stateSample conditions
15H-1H HSQCsample_1not availableExperimental_Conditions
15H-1H TOCSY-HSQCsample_1not availableExperimental_Conditions
15H-1H NOESY-HSQCsample_1not availableExperimental_Conditions
HNCOsample_1not availableExperimental_Conditions
CBCANHsample_1not availableExperimental_Conditions
CBCA(CO)NHsample_1not availableExperimental_Conditions
HCCH-TOCSYsample_1not availableExperimental_Conditions
(HB)CB(CGCD)HD/HEsample_1not availableExperimental_Conditions
13C-edited NOESY-HSQCsample_1not availableExperimental_Conditions

Software:

XEASY v1.5 - Resonance assignment, NOE peak volume measurement

NMR spectrometers:

  • Varian Unity 600 MHz
  • Varian UnityPlus 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

SWISS-PROT P00171
REF NP_776458
PRF 1803548B 1106188A
GenBank ABQ12619 AAI08114 AAC14455
EMBL CAA31949
PDB
BMRB 6131 4810 4809 4808 4807 4806 4805 4804 4803 294 1324 1323