BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5753

Title: Towards an Understanding of the Poliovirus Replication Complex: The Solution Structure of the Soluble Domain of the Poliovirus 3A Protein   PubMed: 12823963

Authors: Strauss, D.; Glustrom, L.; Wuttke, D.

Citation: Strauss, D.; Glustrom, L.; Wuttke, D.. "Towards an Understanding of the Poliovirus Replication Complex: The Solution Structure of the Soluble Domain of the Poliovirus 3A Protein"  J. Mol. Biol. 330, 225-234 (2003).

Assembly members:
Poliovirus 3A, polymer, 60 residues, Formula weight is not available

Natural source:   Common Name: Poliovirus   Taxonomy ID: 138953   Superkingdom: Viruses   Kingdom: not available   Genus/species: Enterovirus poliovirus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Poliovirus 3A: MGPLQYKDLKIDIKTSPPPE CINDLLQAVDSQEVRDYCEK KGWIVNITSQVQTERNINRA

Data sets:
Data typeCount
1H chemical shifts390
13C chemical shifts253
15N chemical shifts67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
13A monomer, chain A1
23A monomer, chain B1

Entities:

Entity 1, 3A monomer, chain A 60 residues - Formula weight is not available

1   METGLYPROLEUGLNTYRLYSASPLEULYS
2   ILEASPILELYSTHRSERPROPROPROGLU
3   CYSILEASNASPLEULEUGLNALAVALASP
4   SERGLNGLUVALARGASPTYRCYSGLULYS
5   LYSGLYTRPILEVALASNILETHRSERGLN
6   VALGLNTHRGLUARGASNILEASNARGALA

Samples:

sample_1: Poliovirus 3A, [U-95% 13C; U-99% 15N], 1.5 mM; potassium phosphate 20 mM; NaCl 50 mM; D2O 100%

sample_2: Poliovirus 3A, [U-95% 13C;U-99% 15N], 2.0 mM; potassium phosphate 20 mM; NaCl 50 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 7.0; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated select/filter NOESYnot availablenot availablenot available

Software:

VNMR v6.1a - collection

NMRPipe v1.0 - processing

ANSIG v3.3 - data analysis

X-PLOR v3.851 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA24445 CAA24446 CAA24456 CAA24461 CAA24465
GB AAA46911 AAA46914 AAL75970 AAM09803 AAM09805
PRF 0707268A 0908283A
REF NP_041277 NP_740474 YP_007353733
SP P03300 P03301 P03302 P06209