BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5758

Title: 1H, 13C and 15N chemical shift assignments for YqgF

Authors: Liu, Dingjiang; Repaka, Prasanti; Taremi, Shane; Wyss, Daniel

Citation: Liu, Dingjiang; Wang, Yu-sen; Wyss, Daniel. "Solution structure of the hypothetical protein YqgF from Escherichia coli reveals an RNAse H fold"  J. Biomol. NMR 27, 389-392 (2003).

Assembly members:
YqgF, polymer, 147 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
YqgF: RGSMADIGSMSGTLLAFDFG TKSIGVAVGQRITGTARPLP AIKAQDGTPDWNIIERLLKE WQPDEIIVGLPLNMDGTEQP LTARARKFANRIHGRFGVEV KLHDERLSTVEARSGLFEQG GYRALNKGKVDSASAVIILE SYFEQGY

Data sets:
Data typeCount
1H chemical shifts778
13C chemical shifts470
15N chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YqgF1

Entities:

Entity 1, YqgF 147 residues - Formula weight is not available

1   ARGGLYSERMETALAASPILEGLYSERMET
2   SERGLYTHRLEULEUALAPHEASPPHEGLY
3   THRLYSSERILEGLYVALALAVALGLYGLN
4   ARGILETHRGLYTHRALAARGPROLEUPRO
5   ALAILELYSALAGLNASPGLYTHRPROASP
6   TRPASNILEILEGLUARGLEULEULYSGLU
7   TRPGLNPROASPGLUILEILEVALGLYLEU
8   PROLEUASNMETASPGLYTHRGLUGLNPRO
9   LEUTHRALAARGALAARGLYSPHEALAASN
10   ARGILEHISGLYARGPHEGLYVALGLUVAL
11   LYSLEUHISASPGLUARGLEUSERTHRVAL
12   GLUALAARGSERGLYLEUPHEGLUGLNGLY
13   GLYTYRARGALALEUASNLYSGLYLYSVAL
14   ASPSERALASERALAVALILEILELEUGLU
15   SERTYRPHEGLUGLNGLYTYR

Samples:

sample_1: YqgF, [U-15N], 0.3 – 1.0 mM; KiPO4 75 mM

sample_2: YqgF, [U-13C; U-15N], 0.5 – 1.0 mM; KiPO4 75 mM

Ex-cond_1: pH: 7.5; temperature: 298 K; ionic strength: 0.45 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HN(CO)not availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONNHnot availablenot availablenot available
13C/15N edited NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

Felix v2000 - process

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 5270
PDB
DBJ BAB37248 BAE77012 BAG78741 BAI27233 BAI32263
EMBL CAP77386 CAQ33259 CAQ90381 CAQ99897 CAR04466
GB AAA69116 AAC75986 AAG58080 AAN44421 AAN81983
REF NP_289521 NP_311852 NP_417424 NP_708714 NP_755410
SP A1AFD5 A7ZR72 A8A489 B1IT58 B1LDF8