BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5771

Title: Solution Structure of the disulphide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins

Authors: Muskett, Frederick; May, Felicity; Westley, Bruce; Feeney, James

Citation: Muskett, Frederick; May, Felicity; Westley, Bruce; Feeney, James. "Solution Structure of the disulphide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins "  Biochemistry ., .-..

Assembly members:
human intestinal trefoil factor 3, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
human intestinal trefoil factor 3: EEYVGLSANQCAVPAKDRVD CGYPHVTPKECNNRGCCFDS RIPGVPWCFKPLQEAECTF

Data sets:
Data typeCount
1H chemical shifts310
15N chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tff3 subunit 11
2tff3 subunit 21

Entities:

Entity 1, tff3 subunit 1 59 residues - Formula weight is not available

1   GLUGLUTYRVALGLYLEUSERALAASNGLN
2   CYSALAVALPROALALYSASPARGVALASP
3   CYSGLYTYRPROHISVALTHRPROLYSGLU
4   CYSASNASNARGGLYCYSCYSPHEASPSER
5   ARGILEPROGLYVALPROTRPCYSPHELYS
6   PROLEUGLNGLUALAGLUCYSTHRPHE

Samples:

sample_1: human intestinal trefoil factor 3, [U-15N], 1.0 – 4.0 mM

conditions_1: pH: 6.8; temperature: 308 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H DQF-COSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
1H-15N HNHAnot availablenot availablenot available
1H-15N (CLEANEX-PM)-FHSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • VARIAN INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA95531 BAB13731
GB AAA59981 AAA83628 AAH17859 AAL28111 AAX36110
REF NP_003217 XP_003823935 XP_004062901 XP_531572
SP Q07654