BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5775

Title: 5'(dCCUCCUU)3':3'(rAGGAGGAAA)5'

Authors: Znosko, B.; Barnes, T.; Krugh, T.; Turner, D.

Citation: Znosko, B.; Barnes, T.; Krugh, T.; Turner, D.. "NMR Studies of DNA Single Strands and DNA:RNA Hybrids With and Without 1-Propynylation at C5 of Oligopyrimidines"  J. Am. Chem. Soc. 125, 6090-6097 (2003).

Assembly members:
RNA chain, polymer, 11 residues, Formula weight is not available
DNA chain, polymer, 7 residues, 308.182 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
RNA chain: UAAAGGAGGAG
DNA chain: CCUCCUU

Data sets:
Data typeCount
1H chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA chain1
2DNA chain2

Entities:

Entity 1, RNA chain 11 residues - Formula weight is not available

1   UAAAGGAGGA
2   G

Entity 2, DNA chain 7 residues - 308.182 Da.

1   DCDCDUDCDCDUDU

Samples:

sample_1: RNA chain 1 mM; DNA chain 1 mM; NaCl 80 mM; phosphate buffer 10 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 80 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K

sample_cond_2: ionic strength: 80 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablenot available

Software:

VNMR v5.2 - collection

FELIX v2000 - data analysis

DISCOVER v95.0 - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links: