BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5776

Title: sPrODN1:RNA [5'-R(AAAGGAGGA)-3'/5'-D(CXXXXXX)-3']

Authors: Znosko, B.; Barnes, T.; Krugh, T.; Turner, D.

Citation: Znosko, B.; Barnes, T.; Krugh, T.; Turner, D.. "NMR Studies of DNA Single Strands and DNA:RNA Hybrids With and Without 1-Propynylation at C5 of Oligopyrimidines"  J. Am. Chem. Soc. 125, 6090-6097 (2003).

Assembly members:
sPrODN1 chain, polymer, 11 residues, Formula weight is not available
DNA chain, polymer, 7 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
sPrODN1 chain: UAAAGGAGGAG
DNA chain: CXXXXXX

Data sets:
Data typeCount
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA chain1
2sPrODN1 chain2

Entities:

Entity 1, RNA chain 11 residues - Formula weight is not available

1   UAAAGGAGGA
2   G

Entity 2, sPrODN1 chain 7 residues - Formula weight is not available

1   DC5PCPDU5PC5PCPDUPDU

Samples:

sample_1: sPrODN1 chain 1 mM; DNA chain 1 mM; NaCl 80 mM; phosphate buffer 10 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 80 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K

sample_cond_2: ionic strength: 80 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablenot available

Software:

VNMR v5.2 - collection

FELIX v2000 - data analysis

DISCOVER v95.0 - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

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