BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5779

Title: The Solution structure of HI1450   PubMed: 14747986

Authors: Parsons, L.; Yeh, Deok; Orban, J.

Citation: Parsons, L.; Yeh, Deok; Orban, J.. "Solution Structure of the highly acidic protein HI1450 from Haemophilus influenzae: a putative double-stranded DNA mimic "  Proteins 54, 375-383 (2004).

Assembly members:
HYPOTHETICAL PROTEIN HI1450, polymer, 110 residues, Formula weight is not available

Natural source:   Common Name: Haemophilus influenzae   Taxonomy ID: 727   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HYPOTHETICAL PROTEIN HI1450: GSHMTTEIKKLDPDTAIDIA YDIFLEMAGENLDPADILLF NLQFEERGGVEFVETADDWE EEIGVLIDPEEYAEVWVGLV NEQDEMDDVFAKFLISHREE DREFHVIWKK

Data sets:
Data typeCount
1H chemical shifts769
13C chemical shifts431
15N chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HI14501

Entities:

Entity 1, HI1450 110 residues - Formula weight is not available

1   GLYSERHISMETTHRTHRGLUILELYSLYS
2   LEUASPPROASPTHRALAILEASPILEALA
3   TYRASPILEPHELEUGLUMETALAGLYGLU
4   ASNLEUASPPROALAASPILELEULEUPHE
5   ASNLEUGLNPHEGLUGLUARGGLYGLYVAL
6   GLUPHEVALGLUTHRALAASPASPTRPGLU
7   GLUGLUILEGLYVALLEUILEASPPROGLU
8   GLUTYRALAGLUVALTRPVALGLYLEUVAL
9   ASNGLUGLNASPGLUMETASPASPVALPHE
10   ALALYSPHELEUILESERHISARGGLUGLU
11   ASPARGGLUPHEHISVALILETRPLYSLYS

Samples:

sample_1: HYPOTHETICAL PROTEIN HI1450, [U-13C; U-15N], 1.0 mM; phosphate 50 mM; NaCl 100 mM; EDTA 0.5 mM; H20 90%; D20 10%

sample_cond_1: pH: 6.75 na; temperature: 298 K; ionic strength: 150 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
CCONHnot availablenot availablenot available
HCCONHnot availablenot availablenot available
HBHAnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBHDnot availablenot availablenot available
CBHEnot availablenot availablenot available

Software:

CNS v1.1 - structure solution, refinement

NMRPipe v5.0.3 - processing

SPARKY v3.106 - data analysis

NOEID v1.0 - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CBW29844 CBY80947 CBY87103
GB AAC23100 AAX88470 ABQ98363 ABQ99322 ADO80886
REF NP_439602 WP_005628170 WP_005636034 WP_005638589 WP_005641855
SP A5UEW7 P44199 Q4QKH7