BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5780

Title: NMR structure of the PYRIN domain of human ASC   PubMed: 14499617

Authors: Liepinsh, Edvards; Barbals, Raitis; Dahl, Edgar; Sharipo, Anatoly; Staub, Eike; Otting, Gottfried

Citation: Liepinsh, Edvards; Barbals, Raitis; Dahl, Edgar; Sharipo, Anatoly; Staub, Eike; Otting, Gottfried. "The Death-domain Fold of the ASC PYRIN Domain, Presenting a Basis for PYRIN/PYRIN Recognition "  J. Mol. Biol. 332, 1155-1163 (2003).

Assembly members:
PYRIN domain, polymer, 103 residues, 10076 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PYRIN domain: MRGSHHHHHHGSMGRARDAI LDALENLTAEELKKFKLKLL SVPLREGYGRIPRGALLSMD ALDLTDKLVSFYLETYGAEL TANVLRDMGLQEMAGQLQAA THQ

Data sets:
Data typeCount
1H chemical shifts669

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PYRIN domain 11

Entities:

Entity 1, PYRIN domain 1 103 residues - 10076 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERMETGLYARGALAARGASPALAILE
3   LEUASPALALEUGLUASNLEUTHRALAGLU
4   GLULEULYSLYSPHELYSLEULYSLEULEU
5   SERVALPROLEUARGGLUGLYTYRGLYARG
6   ILEPROARGGLYALALEULEUSERMETASP
7   ALALEUASPLEUTHRASPLYSLEUVALSER
8   PHETYRLEUGLUTHRTYRGLYALAGLULEU
9   THRALAASNVALLEUARGASPMETGLYLEU
10   GLNGLUMETALAGLYGLNLEUGLNALAALA
11   THRHISGLN

Samples:

sample_1: PYRIN domain 1.0 mM; NaCl 0.05 M

Ex-cond_1: pH: 3.7; temperature: 301 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
w1-decoupled NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

XEASY v3.0 - peak assignment

OPAL v2.6 - energy minimization

DYANA v2.8 - structure calculation

NMR spectrometers:

  • Varian Unity 800 MHz

Related Database Links:

BMRB 18608
PDB
DBJ BAA87339 BAG37041 BAG73625
GB AAF99665 AAG01187 AAG01188 AAG30286 AAH13569
REF NP_037390 NP_660183 XP_001158625 XP_001158687 XP_002826422
SP Q9ULZ3