BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5781

Title: The Solution Structure of a DNA.RNA Duplex Containing 5-Propynyl U and C Comparison with 5-Me Modifications   PubMed: 12736318

Authors: Gyi, J.; Gao, D.; Conn, G.; Trent, J.; Brown, T.; Lane, A.

Citation: Gyi, J.; Gao, D.; Conn, G.; Trent, J.; Brown, T.; Lane, A.. "The Solution Structure of a DNA.RNA Duplex Containing 5-Propynyl U and C; Comparison with 5-Me Modifications"  Nucleic Acids Res. 31, 2683-2693 (2003).

Assembly members:
DNA.RNA_hybrid, polymer, 10 residues, 6000 Da.
DNA.RNA_hybrid, polymer, 10 residues, 6000 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA.RNA_hybrid: GCXXCXCXXC
DNA.RNA_hybrid: GAAGAGAAGC

Data sets:
Data typeCount
1H chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Propynyl_DNA.RNA_hybrid chain A1
2Propynyl_DNA.RNA_hybrid chain B2

Entities:

Entity 1, Propynyl_DNA.RNA_hybrid chain A 10 residues - 6000 Da.

1   DGDCDUDUDCDUDCDUDUDC

Entity 2, Propynyl_DNA.RNA_hybrid chain B 10 residues - 6000 Da.

1   GAAGAGAAGC

Samples:

sample_1: DNA.RNA_hybrid 1 mM; DNA.RNA_hybrid 1 mM; D2O 100%; KCL 100 mM

sample_2: DNA.RNA_hybrid 1 mM; DNA.RNA_hybrid 1 mM; H2O 90%; D2O 10%; KCL 100 mM

sample_cond_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 ATM; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availablesample_cond_1
COSYnot availablenot availablesample_cond_1
2Q COSY(INADEQUATE)not availablenot availablesample_cond_1

Software:

AMBER v7.0 - refinement

FELIX v95.0 - processing

NUCFIT v0.0012 - data analysis

PFIT v1.1 - data analysis

NMR spectrometers:

  • VARIAN UNITY PLUS 600 MHz
  • VARIAN UNITY 500 MHz

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