BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5786

Title: Solution Structure of Neural Cadherin Prodomain   PubMed: 14739643

Authors: Koch, A.; Farooq, A.; Shan, W.; Gruzglin, G.; Zeng, L.; Colman, D.; Zhou, M.-M.

Citation: Koch, Alexander; Farooq, Amjad; Zeng, Lei; Colman, David; Zhou, M.-M.. "Letter to the Editor: 1H, 15N and 13C resonance assignments for the N-cadherin prodomain"  J. Biomol. NMR 28, 87-88 (2004).

Assembly members:
N-cadherin prodomain, polymer, 159 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
N-cadherin prodomain: GHHHHHHHHHHSSGHIDDDD KHMEASGEIALCKTGFPEDV YSAVLPKDVHEGQPLLNVKF SNCNRKRKVQYESSEPADFK VDEDGTVYAVRSFPLTAEQA KFLIYAQDKETQEKWQVAVN LSREPTLTEEPMKEPHEIEE IVFPRQLAKHSGALQRQKR

Data sets:
Data typeCount
13C chemical shifts416
15N chemical shifts118
1H chemical shifts621

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-cadherin, prodomain1

Entities:

Entity 1, N-cadherin, prodomain 159 residues - Formula weight is not available

1   GLYHISHISHISHISHISHISHISHISHIS
2   HISSERSERGLYHISILEASPASPASPASP
3   LYSHISMETGLUALASERGLYGLUILEALA
4   LEUCYSLYSTHRGLYPHEPROGLUASPVAL
5   TYRSERALAVALLEUPROLYSASPVALHIS
6   GLUGLYGLNPROLEULEUASNVALLYSPHE
7   SERASNCYSASNARGLYSARGLYSVALGLN
8   TYRGLUSERSERGLUPROALAASPPHELYS
9   VALASPGLUASPGLYTHRVALTYRALAVAL
10   ARGSERPHEPROLEUTHRALAGLUGLNALA
11   LYSPHELEUILETYRALAGLNASPLYSGLU
12   THRGLNGLULYSTRPGLNVALALAVALASN
13   LEUSERARGGLUPROTHRLEUTHRGLUGLU
14   PROMETLYSGLUPROHISGLUILEGLUGLU
15   ILEVALPHEPROARGGLNLEUALALYSHIS
16   SERGLYALALEUGLNARGGLNLYSARG

Samples:

sample_1: N-cadherin prodomain, [U-13C; U-15N], 1 mM; Phosphate 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

NMRPipe - processing

X-PLOR - structure solution

xwinnmr - collection

ARIA - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links: