BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5798

Title: Solution structure of Protein yrbA from Escherichia Coli: Northeast Structural Genomics Consortium target ER115

Authors: Gurla, S.; Huang, J.; Acton, T.; Shastry, R.; Chiang, Y.-W.; Montelione, G.

Citation: Gurla, S.; Huang, J.; Acton, T.; Shastry, R.; Chiang, Y.-W.; Montelione, G.. "Solution structure of Protein yrbA from Escherichia Coli: Northeast Structural Genomics Consortium target ER115 "  . ., .-..

Assembly members:
yrbA, polymer, 97 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
yrbA: MIEDPMENNEIQSVLMNALS LQEVHVSGDGSHFQVIAVGE LFDGMSRVKKQQTVYGPLME YIADNRIHAVSIKAYTPAEW ARDRKLNGFLEHHHHHH

Data sets:
Data typeCount
1H chemical shifts656
13C chemical shifts384
15N chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yrbA1

Entities:

Entity 1, yrbA 97 residues - Formula weight is not available

1   METILEGLUASPPROMETGLUASNASNGLU
2   ILEGLNSERVALLEUMETASNALALEUSER
3   LEUGLNGLUVALHISVALSERGLYASPGLY
4   SERHISPHEGLNVALILEALAVALGLYGLU
5   LEUPHEASPGLYMETSERARGVALLYSLYS
6   GLNGLNTHRVALTYRGLYPROLEUMETGLU
7   TYRILEALAASPASNARGILEHISALAVAL
8   SERILELYSALATYRTHRPROALAGLUTRP
9   ALAARGASPARGLYSLEUASNGLYPHELEU
10   GLUHISHISHISHISHISHIS

Samples:

sample_1: yrbA, [U-13C; U-15N], 0.8 mM; NH4OAc 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; azide 0.02%; H2O 95%; D2O 5%

sample_cond_1: pH: 5.5; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

VNMR v6.1B - collection

NMRPipe v2.1 - processing

SPARKY v3.106 - data analysis

AUTOASSIGN v1.9 - data analysis

AUTOSTRUCTURE v1.1.2 - data analysis

HYPER v3.2 - structure solution

DYANA v1.5 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB37492 BAE77234 BAG78998 BAI27468 BAI32647
EMBL CAP77650 CAQ33523 CAQ90660 CAR00152 CAR04800
GB AAA57991 AAC76222 AAF21251 AAG58324 AAN44696
REF NP_289764 NP_312096 NP_417657 NP_708989 NP_755814
SP P0A9W6 P0A9W7 P0A9W8