BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5808

Title: Chemical shift assignments and relaxation parameters for the PAH2 domain of mSin3B bound to an extended SID of Mad1   PubMed: 14705930

Authors: van Ingen, Hugo; Jansen, Jaap; Spronk, Chris; Vuister, Geerten

Citation: van Ingen, Hugo; Lasonder, Edwin; Jansen, Jaap; Kaan, Anita; Spronk, Chris; Stunnenberg, Henk; Vuister, Geerten. "Extension of the binding motif of the Sin3 Interacting Domain of the mad family proteins"  Biochemistry 43, 46-54 (2004).

Assembly members:
PAH2 domain, polymer, 105 residues, Formula weight is not available
Mad1, polymer, 24 residues, 2973 Da.

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PAH2 domain: ESDSVEFNNAISYVNKIKTR FLDHPEIYRSFLEILHTYQK EQLHTKGRPFRGMSEEEVFT EVANLFRGQEDLLSEFGQFL PEAKRSLFTGNGSAEMNSGQ KNEEK
Mad1: VRMNIQMLLEAADYLERRER EAEH

Data sets:
Data typeCount
1H chemical shifts552
13C chemical shifts269
15N chemical shifts90
T1 relaxation values78
order parameters77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAH2 domain of mSin3B1
2SID domain of Mad12

Entities:

Entity 1, PAH2 domain of mSin3B 105 residues - Formula weight is not available

1   GLUSERASPSERVALGLUPHEASNASNALA
2   ILESERTYRVALASNLYSILELYSTHRARG
3   PHELEUASPHISPROGLUILETYRARGSER
4   PHELEUGLUILELEUHISTHRTYRGLNLYS
5   GLUGLNLEUHISTHRLYSGLYARGPROPHE
6   ARGGLYMETSERGLUGLUGLUVALPHETHR
7   GLUVALALAASNLEUPHEARGGLYGLNGLU
8   ASPLEULEUSERGLUPHEGLYGLNPHELEU
9   PROGLUALALYSARGSERLEUPHETHRGLY
10   ASNGLYSERALAGLUMETASNSERGLYGLN
11   LYSASNGLUGLULYS

Entity 2, SID domain of Mad1 24 residues - 2973 Da.

1   VALARGMETASNILEGLNMETLEULEUGLU
2   ALAALAASPTYRLEUGLUARGARGGLUARG
3   GLUALAGLUHIS

Samples:

sample_1: Mad1 1.3 mM; PAH2 domain, [U-13C; U-15N], 1.3 mM; K2HPO4/KH2PO4 buffer 50 mM

Ex-cond_1: pH: 6.3; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CBCACONHsample_1not availableEx-cond_1
(H)CCH-TOCSYsample_1not availableEx-cond_1
15N NOESYsample_1not availableEx-cond_1
13C NOESYsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian UnityInova 500 MHz
  • Varian UnityInova 600 MHz
  • Varian UnityInova 750 MHz
  • Varian UnityInova 800 MHz

Related Database Links:

PDB
DBJ BAG35605 BAG73371 BAH13406 BAG35605
EMBL CAG38734 CAG46493 CAG46493 CAG38734
GB AAA36194 AAH69377 AAH69433 AAH98396 AAI13532
REF NP_001179309 NP_001189442 NP_001189443 NP_001247787 NP_002348 XP_592508 XP_525776 XP_001493078 XP_001098088 NP_002348
SP Q05195
TPG DAA24557
SWISS-PROT Q05195
GenBank AAI13532 AAH98396 AAH69433 AAH69377 AAA36194
BMRB 5457 4635