BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5817

Title: NMR Structure of a Prototype LNR Module from Human Notch1   PubMed: 12795601

Authors: Vardar, D.; North, C.; Sanchez-Irizarry, C.; Aster, J.; Blacklow, S.

Citation: Vardar, D.; North, C.; Sanchez-Irizarry, C.; Aster, J.; Blacklow, S.. "Nuclear Magnetic Resonance Structure of a Prototype Lin12-Notch Repeat Module from Human Notch1 "  Biochemistry 42, 7061-7067 (2003).

Assembly members:
Neurogenic locus notch homolog protein 1, polymer, 35 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Neurogenic locus notch homolog protein 1: EEACELPECQEDAGNKVCSL QCNNHACGWDGGDCS

Data sets:
Data typeCount
1H chemical shifts177
13C chemical shifts82
15N chemical shifts40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Intersectin 21
2CALCIUM ION2

Entities:

Entity 1, Intersectin 2 35 residues - Formula weight is not available

1   GLUGLUALACYSGLULEUPROGLUCYSGLN
2   GLUASPALAGLYASNLYSVALCYSSERLEU
3   GLNCYSASNASNHISALACYSGLYTRPASP
4   GLYGLYASPCYSSER

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Neurogenic locus notch homolog protein 1, [U-15N], 1 mM; calcium salt 10 mM; DSS 0.5 mM; H2O 90%; D2O 10%

sample_2: Neurogenic locus notch homolog protein 1, [U-13C; U-15N], 1 mM; calcium salt 10 mM; DSS 0.5 mM; H2O 90%; D2O 10%

sample_3: Neurogenic locus notch homolog protein 1, [U-15N], 1.5 mM; calcium salt 10 mM; DSS 0.5 mM; PIPES, [U-2H], 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5; temperature: 298 K; ionic strength: 10 mM; pressure: 1 atm

sample_cond_2: pH: 7.0; temperature: 283 K; ionic strength: 60 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
15N-HSQCnot availablenot availablenot available
15N-HMQC-Jnot availablenot availablenot available

Software:

XWINNMR - collection

NMRPipe - processing

NmrView v5.0.4 - data analysis

CNS v1.1 - structure solution, structure refinement

NMR spectrometers:

  • Varian UNITY 400 MHz

Related Database Links:

PDB
DBJ BAD93110
EMBL CAA40667
GB AAA60614 AAG33848 EAW88241 EDL93491 EGW12778
REF NP_001099191 NP_060087 XP_002808138 XP_003473037 XP_003510910
SP G3I6Z6 P46531 Q07008