BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5818

Title: The solution structure of YbcJ from E. coli reveals a recently discovered alfaL motif involved in RNA-binding   PubMed: 12837795

Authors: Volpon, L.; Lievre, C.; Osborne, M.; Gandhi, S.; Iannuzzi, P.; Larocque, R.; Matte, A.; Cygler, M.; Gehring, K.; Ekiel, I.

Citation: Volpon, L.; Lievre, C.; Osborne, M.; Gandhi, S.; Iannuzzi, P.; Larocque, R.; Cygler, M.; Gehring, K.; Ekiel, I.. "The Solution Structure of YbcJ from Escherichia coli Reveals a Recently Discovered alphaL Motif Involved in RNA Binding "  J. Bacteriol. 185, 4204-4210 (2003).

Assembly members:
YbcJ, polymer, 79 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
YbcJ: GSMIHRMSNMATFSLGKHPH VELCDLLKLEGWSESGAQAK IAIAEGQVKVDGAVETRKRC KIVAGQTVSFAGHSVQVVA

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YbcJ1

Entities:

Entity 1, YbcJ 79 residues - Formula weight is not available

1   GLYSERMETILEHISARGMETSERASNMET
2   ALATHRPHESERLEUGLYLYSHISPROHIS
3   VALGLULEUCYSASPLEULEULYSLEUGLU
4   GLYTRPSERGLUSERGLYALAGLNALALYS
5   ILEALAILEALAGLUGLYGLNVALLYSVAL
6   ASPGLYALAVALGLUTHRARGLYSARGCYS
7   LYSILEVALALAGLYGLNTHRVALSERPHE
8   ALAGLYHISSERVALGLNVALVALALA

Samples:

sample_1: YbcJ, [U-13C; U-15N], 4 – 7 mM; phosphate buffer 50 mM; NaCl 300 mM; DTT 15 mM; sodium azide 1 mM; H2O 90%; D2O 10%

sample_2: YbcJ, [U-13C; U-15N], 4 – 7 mM; phosphate buffer 50 mM; NaCl 300 mM; DTT 15 mM; sodium azide 1 mM; Pf1 phage 8 mg/mL; H2O 90%; D2O 10%

sample_cond_1: pH: 6.8; temperature: 313 K; ionic strength: 350 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
IPAP-HSQnot availablenot availablesample_cond_1

Software:

GIFA v4.31 - processing

XWINNMR v2.1 - collection

XEASY v1.3.13 - data analysis

TALOS v2003.027.13.05 - refinement

ARIA v1.1 - refinement

CNS v1.1 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAB34013 BAE76305 BAG76077 BAI23895 BAI29367
EMBL CAP75058 CAQ31000 CAQ88117 CAQ97402 CAR01873
GB AAB40281 AAC73630 AAG54885 AAN42113 AAN79121
REF NP_286277 NP_308617 NP_415061 NP_706406 NP_752577
SP P0AAS7 P0AAS8