BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5823

Title: 1H, 15N and 13C assignments of single-stranded DNA binding domains from the 70 kDa subunit of Human Replication Protein A   PubMed: 14755166

Authors: Bhattacharya, Shibani; Arunkumar, Alphonse; Sullivan, Shannon; Botuyan, Maria-Victoria; Arrowsmith, Cheryl; Chazin, Walter

Citation: Bhattacharya, Shibani; Arunkumar, Alphonse; Sullivan, Shannon; Botuyan, Maria-Victoria; Arrowsmith, Cheryl; Chazin, Walter. "Letter to the Editor: 1H, 13C and 15N assignments of single-stranded DNA binding domains from the 70 kDa subunit of Human Replication Protein A"  J. Biomol. NMR 27, 195-196 (2004).

Assembly members:
single chain biopolymer, polymer, 242 residues, Formula weight is not available

Natural source:   Common Name: 9606   Taxonomy ID: Human   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
single chain biopolymer: QSKVVPIASLTPYQSKWTIC ARVTNKSQIRTWSNSRGEGK LFSLELVDESGEIRATAFNE QVDKFFPLIEVNKVYYFSKG TLKIANKQFTAVKNDYEMTF NNETSVMPCEDDHHLPTVQF DFTGIDDLENKSKDSLVDII GICKSYEDATKITVRSNNRE VAKRNIYLMDTSGKVVTATL WGEDADKFDGSRQPVLAIKG ARVSDFGGRSLSVLSSSTII ANPDIPEAYKLRGWFDAEGQ AL

Data sets:
Data typeCount
1H chemical shifts1634
13C chemical shifts1036
15N chemical shifts248

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RPA70(181-422)1

Entities:

Entity 1, RPA70(181-422) 242 residues - Formula weight is not available

1   GLNSERLYSVALVALPROILEALASERLEU
2   THRPROTYRGLNSERLYSTRPTHRILECYS
3   ALAARGVALTHRASNLYSSERGLNILEARG
4   THRTRPSERASNSERARGGLYGLUGLYLYS
5   LEUPHESERLEUGLULEUVALASPGLUSER
6   GLYGLUILEARGALATHRALAPHEASNGLU
7   GLNVALASPLYSPHEPHEPROLEUILEGLU
8   VALASNLYSVALTYRTYRPHESERLYSGLY
9   THRLEULYSILEALAASNLYSGLNPHETHR
10   ALAVALLYSASNASPTYRGLUMETTHRPHE
11   ASNASNGLUTHRSERVALMETPROCYSGLU
12   ASPASPHISHISLEUPROTHRVALGLNPHE
13   ASPPHETHRGLYILEASPASPLEUGLUASN
14   LYSSERLYSASPSERLEUVALASPILEILE
15   GLYILECYSLYSSERTYRGLUASPALATHR
16   LYSILETHRVALARGSERASNASNARGGLU
17   VALALALYSARGASNILETYRLEUMETASP
18   THRSERGLYLYSVALVALTHRALATHRLEU
19   TRPGLYGLUASPALAASPLYSPHEASPGLY
20   SERARGGLNPROVALLEUALAILELYSGLY
21   ALAARGVALSERASPPHEGLYGLYARGSER
22   LEUSERVALLEUSERSERSERTHRILEILE
23   ALAASNPROASPILEPROGLUALATYRLYS
24   LEUARGGLYTRPPHEASPALAGLUGLYGLN
25   ALALEU

Samples:

Sample_1: single chain biopolymer, [U-15N; U-13C], 0.5 – 1.0 mM; tris-d11 20 mM; Potassium Chloride 50 mM; Magnesium Chloride 10 mM; DTT 2 mM; Sodium Azide 0.01%; H2O 90%; D2O 10%

sample_cond_1: pH: 7.2; temperature: 298 K; ionic strength: 0.080 M

Experiments:

NameSampleSample stateSample conditions
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
H(CCO)NH-TOCSYnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available

Software:

FELIX v2000 -

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16754 5822
PDB
DBJ BAD92969 BAF82393 BAG10883
EMBL CAH92206
GB AAA36584 AAH18126 AAS94324 ADR83314 AIC58576
REF NP_001126290 NP_002936 XP_002747872 XP_002800290 XP_003277864
SP P27694 Q5R7Q4