BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5824

Title: Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein (oxidized form)   PubMed: 14872133

Authors: Vakonakis, Ioannis; LiWang, Andy

Citation: Vakonakis, Ioannis; LiWang, Andy. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of the C-terminal domain of KaiA, a circadian clock protein "  J. Biomol. NMR 28, 403-404 (2004).

Assembly members:
Thermosynechococcus KaiA, polymer, 107 residues, 12583 Da.

Natural source:   Common Name: Thermosynechococcus elongatus   Taxonomy ID: 146786   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Thermosynechococcus KaiA: AMARMSPADKRKLLDELRSI YRTIVLEYFNTDAKVNERID EFVSKAFFADISVSQVLEIH VELMDTFSKQLKLEGRSEDI LLDYRLTLIDVIAHLCEMYR RSIPREV

Data sets:
Data typeCount
1H chemical shifts789
13C chemical shifts392
15N chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ThKaiA180C subunit 11
2ThKaiA180C subunit 21

Entities:

Entity 1, ThKaiA180C subunit 1 107 residues - 12583 Da.

1   ALAMETALAARGMETSERPROALAASPLYS
2   ARGLYSLEULEUASPGLULEUARGSERILE
3   TYRARGTHRILEVALLEUGLUTYRPHEASN
4   THRASPALALYSVALASNGLUARGILEASP
5   GLUPHEVALSERLYSALAPHEPHEALAASP
6   ILESERVALSERGLNVALLEUGLUILEHIS
7   VALGLULEUMETASPTHRPHESERLYSGLN
8   LEULYSLEUGLUGLYARGSERGLUASPILE
9   LEULEUASPTYRARGLEUTHRLEUILEASP
10   VALILEALAHISLEUCYSGLUMETTYRARG
11   ARGSERILEPROARGGLUVAL

Samples:

sample_1: Thermosynechococcus KaiA, [U-99% 13C; U-99% 15N], 1.2 mM; Na2HPO4 20.0 mM; NaCl 20.0 mM

sample_2: Thermosynechococcus KaiA 1.2 mM; Na2HPO4 20.0 mM; NaCl 20.0 mM

cond_1: pH: 7.0 na; temperature: 323 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
H(C)CH-COSYnot availablenot availablenot available
4D 13C-13C edited NOESYnot availablenot availablenot available

Software:

NMRPipe v2.1 Rev 2002.044.17.08 - data processing

PIPP v4.2.6 - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 5825
PDB
DBJ BAB85983 BAC08033 BAD21221
REF NP_681271 WP_011056332
SP Q6L8K1 Q79V62