BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5836

Title: Backbone 1H, 13C, 15N chemical shifts and coupling constants of Yersinia pseudotuberculosis-derived mitogen

Authors: Liew, Chu Wai; Donadini, Roberta; Fields, Barry; Mackay, Joel

Citation: Liew, Chu Wai; Donadini, Roberta; Fields, Barry; Mackay, Joel. "Backbone 1H, 13C, 15N chemical shifts and coupling constants of Yersinia pseudotuberculosis-derived mitogen"  The BMRB entry is the only known published source for the data..

Assembly members:
Yersinia pseudotuberculosis-derived mitogen, polymer, 119 residues, Formula weight is not available

Natural source:   Common Name: Yersinia pseudotuberculosis   Taxonomy ID: 633   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Yersinia pseudotuberculosis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Yersinia pseudotuberculosis-derived mitogen: LRIPNIATYTGTIQGKGEVC IIGNKEGKTRGGELYAVLHS TNVNADMTLILLRNVGGNGW GEIKRNDIDKPLKYEDYYTS GLSWIWKIKNNSSETSNYSL DATVHDDKEDSDVLTKCPV

Data sets:
Data typeCount
1H chemical shifts709
13C chemical shifts154
15N chemical shifts91
coupling constants28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YPM1

Entities:

Entity 1, YPM 119 residues - Formula weight is not available

1   LEUARGILEPROASNILEALATHRTYRTHR
2   GLYTHRILEGLNGLYLYSGLYGLUVALCYS
3   ILEILEGLYASNLYSGLUGLYLYSTHRARG
4   GLYGLYGLULEUTYRALAVALLEUHISSER
5   THRASNVALASNALAASPMETTHRLEUILE
6   LEULEUARGASNVALGLYGLYASNGLYTRP
7   GLYGLUILELYSARGASNASPILEASPLYS
8   PROLEULYSTYRGLUASPTYRTYRTHRSER
9   GLYLEUSERTRPILETRPLYSILELYSASN
10   ASNSERSERGLUTHRSERASNTYRSERLEU
11   ASPALATHRVALHISASPASPLYSGLUASP
12   SERASPVALLEUTHRLYSCYSPROVAL

Samples:

sample_1: Yersinia pseudotuberculosis-derived mitogen, [U-95% 13C; U-90% 15N], 0.5 mM

sample_2: Yersinia pseudotuberculosis-derived mitogen 0.5 mM

Condition_1: pH: 6.5 na; temperature: 298 K; ionic strength: 0 M

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

XWINNMR v2.6 - raw spectral data processing

XEASY v1.3.13 - data analysis, assignments

DYANA v1.5 - structure refinement

ARIA v1.1.2 - structure refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA07528 BAA07619
GB AAK28542 AAL02230 ABS46381
REF WP_012105075 WP_038824931 YP_001400833