BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5839

Title: Characterization of the overall and local dynamics of a protein with intermediate rotational anisotropy: Differentiating between conformational exchange and anisotropic diffusion in the B3 domain of protein G   PubMed: 12975584

Authors: Hall, Jennifer; Fushman, David

Citation: Hall, Jennifer; Fushman, David. "Characterization of the Overall and Local Dynamics of a Protein with Intermediate Rotational Anisotropy: Differentiating Between Conformational Exchange and Anisotropic Diffusion in the B3 Domain of Protein G "  J. Biomol. NMR 27, 261-275 (2003).

Assembly members:
B3 domain of Streptococcal protein G, polymer, 56 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1301   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptococcus not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
B3 domain of Streptococcal protein G: MQYKLVINGKTLKGETTTKA VDAETAEKAFKQYANDNGVD GVWTYDDATKTFTVTE

Data sets:
Data typeCount
T1 relaxation values51
T2 relaxation values51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GB3 subunit 11

Entities:

Entity 1, GB3 subunit 1 56 residues - Formula weight is not available

1   METGLNTYRLYSLEUVALILEASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRLYSALA
3   VALASPALAGLUTHRALAGLULYSALAPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYVALTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Samples:

sample_one: B3 domain of Streptococcal protein G, [U-15N], 1.8 mM

sample_conditions_one: pH: 5.8; temperature: 297 K

Experiments:

NameSampleSample stateSample conditions
15N R1sample_onenot availablesample_conditions_one
15N R2sample_onenot availablesample_conditions_one
15N-1H NOEsample_onenot availablesample_conditions_one

Software:

XWINNMR v3.0 - NMR data processing

RELAXFIT - curve fitting, error estimation

AUTOPICK - peak picking, intensity calculation

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 15283 18531 2575
PDB
GB AAA26921 AAB27024 ABL60854 ABL60859 ABO76907